Distinct Gene Expression and Epigenetic Signatures in Hepatocyte-like Cells Produced by Different Strategies from the Same Donor

doi:10.1016/j.stemcr.2017.10.019

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	Distinct Gene Expression and Epigenetic Signatures in Hepatocyte-like Cells Produced by Different Strategies from the Same Donor
	Gao, Yimeng 1; Zhang, Xiaoran 7; Zhang, Ludi 1; Cen, Jin 1; Ni, Xuan 6; Liao, Xiaoying 6; Yang, Chenxi 5; Li, Ying 7; Chen, Xiaotao 1; Zhang, Zhao 1
刊名	STEM CELL REPORTS
	2017-12
卷号	9 期号:6 页码:1813-1824
ISSN号	2213-6711
DOI	10.1016/j.stemcr.2017.10.019
文献子类	Article
英文摘要	Hepatocyte-like cells (HLCs) can be generated through directed differentiation or transdifferentiation. Employing two strategies, we generated induced pluripotent stem cell (iPSC)-HLCs and hiHeps from the same donor cell line. Both types of HLCs clustered distinctly from each other during gene expression profiling. In particular, differences existed in gene expression for phase II drug metabolism and lipid accumulation, underpinned by H3K27 acetylation status in iPSC-HLCs and hiHeps. While distinct phenotypes were achieved in vitro, both types of HLCs demonstrated similar phenotypes following transplantation into Fah-deficient mice. In conclusion, functional HLCs can be obtained from the same donor using two strategies. Global gene expression defined the differences between those populations in vitro. Importantly, both HLCs displayed partial but markedly improved hepatic function following transplantation in vivo, demonstrating plasticity and the potential for cell-based modeling in the dish and cell-based therapy in the future.
资助项目	CAS-CSIRO[153D31KYSB20160247] ; CAS-NWO Programme[153D31KYSB20150085] ; National Natural Science Foundation of China (NSFC)[31630044] ; National Natural Science Foundation of China (NSFC)[31601186] ; National Natural Science Foundation of China (NSFC)[81471948] ; National Natural Science Foundation of China (NSFC)[31771431] ; National Natural Science Foundation of China (NSFC)[31271354] ; National Natural Science Foundation of China (NSFC)[31401263] ; Ministry of Science and Technology of China (MOST)[2013CB967103] ; Science and Technology Commission of Shanghai Municipality (STCSM)[16JC1400202] ; Science and Technology Commission of Shanghai Municipality (STCSM)[15JC1400200] ; Shanghai Zhangjiang National Independent Innovation Demonstration Zone Stem Cells Translational Medicine Industry Base Project[ZJ2014-ZD-002] ; UK Regenerative Medicine Platform award[MR/L022974/1]
WOS关键词	PLURIPOTENT STEM-CELLS ; DEFINED FACTORS ; FUNCTIONAL HEPATOCYTES ; HUMAN FIBROBLASTS ; HEPATIC ENDODERM ; HUMAN LIVER ; TRANSPLANTATION ; GENERATION ; MICE ; INDUCTION
WOS研究方向	Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000417690600007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272372]
专题	药物安全性评价中心
通讯作者	Wei, Gang; Hui, Lijian
作者单位	1.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai 200031, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Shanghai 200030, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Univ Edinburgh, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland; 5.East China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai 201210, Peoples R China; 7.Univ Chinese Acad Sci, Shanghai Inst Biol Sci, Collaborat Innovat Ctr Genet & Dev Biol,Chinese A, CAS Key Lab Computat Biol,CAS MPG Partner Inst Co, Shanghai 200031, Peoples R China;
推荐引用方式 GB/T 7714	Gao, Yimeng,Zhang, Xiaoran,Zhang, Ludi,et al. Distinct Gene Expression and Epigenetic Signatures in Hepatocyte-like Cells Produced by Different Strategies from the Same Donor[J]. STEM CELL REPORTS,2017,9(6):1813-1824.
APA	Gao, Yimeng.,Zhang, Xiaoran.,Zhang, Ludi.,Cen, Jin.,Ni, Xuan.,...&Hui, Lijian.(2017).Distinct Gene Expression and Epigenetic Signatures in Hepatocyte-like Cells Produced by Different Strategies from the Same Donor.STEM CELL REPORTS,9(6),1813-1824.
MLA	Gao, Yimeng,et al."Distinct Gene Expression and Epigenetic Signatures in Hepatocyte-like Cells Produced by Different Strategies from the Same Donor".STEM CELL REPORTS 9.6(2017):1813-1824.