Effect of microenvironment on beta-site amyloid precursor protein cleaving enzyme 1 expression in PC12 Cells

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	Effect of microenvironment on beta-site amyloid precursor protein cleaving enzyme 1 expression in PC12 Cells
	Yang Huanqing 3; He Xiang 1; Du Xiaoguang 1; Xiao Shifu 3; Wang Tao 3
刊名	Journal of Shanghai Jiaotong University .Medical Science
	2018
卷号	38 期号:9 页码:1005-1012
关键词	Alzheimer's disease microenvironment PC12 cell beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) amyloid-beta peptide (Abeta)
ISSN号	1674-8115
其他题名	微环境变化对PC12细胞淀粉样前体蛋白beta位点裂解酶1表达的影响
文献子类	Article
英文摘要	Objective · To investigate the effect of microenvironment on the expression of beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) in PC12 cells. Methods · The PC12 cells were respectively treated with thapsigargin (Tg), lipopolysaccharide (LPS), H_2O_2, hypoxia incubator and hypoglycemic medium to produce mild endoplasmic reticulum stress (ERS), inflammatory environment, mild oxidative stress, hypoxia and low glucose conditions. Western blotting and fluorescence quantitative PCR were used to detect the expression of BACE1 protein and BACE1 mRNA. Results · After PC12 cells were treated with Tg (0.13 mumol/L) for 12 h or LPS (0.01 mg/mL) for 36 h, BACE1 protein and mRNA levels were significantly up-regulated (P<0.05). No significant changes were observed in the expression of BACE1 protein and mRNA of the PC12 cells treated with H_2O_2 (0.13 mmol/L or 0.25 mmol/L) for 12 h, under hypoxic or hypoglycemic environment (P>0.05). Conclusion · Mild ERS and inflammatory condition can up-regulate BACE1 mRNA and protein expression in PC12 cells.
资助项目	国家自然科学基金[00000000] ; 上海市市级医疗卫生优秀学科带头人培养计划[00000000] ; 上海浦江人才计划[00000000] ; 上海市教育委员会高峰高原学科项目[00000000]
WOS研究方向	Neurosciences & Neurology (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:6343222
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269152]
专题	药物安全性评价中心
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, State Key Laboratory of Drug Research, Shanghai 201203, China. 2.Shanghai Jiao Tong University, Shanghai 200030, China.; 3.Department of Geriatric Psychiatry, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine;
推荐引用方式 GB/T 7714	Yang Huanqing,He Xiang,Du Xiaoguang,et al. Effect of microenvironment on beta-site amyloid precursor protein cleaving enzyme 1 expression in PC12 Cells[J]. Journal of Shanghai Jiaotong University .Medical Science,2018,38(9):1005-1012.
APA	Yang Huanqing,He Xiang,Du Xiaoguang,Xiao Shifu,&Wang Tao.(2018).Effect of microenvironment on beta-site amyloid precursor protein cleaving enzyme 1 expression in PC12 Cells.Journal of Shanghai Jiaotong University .Medical Science,38(9),1005-1012.
MLA	Yang Huanqing,et al."Effect of microenvironment on beta-site amyloid precursor protein cleaving enzyme 1 expression in PC12 Cells".Journal of Shanghai Jiaotong University .Medical Science 38.9(2018):1005-1012.