Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2

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	Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2
	Xie Junling 2; Zhang Yue 2; Huang Xiaojie 2; Zang Yi1
刊名	Acta Anatomica Sinica
	2012
卷号	43 期号:5 页码:581-587
关键词	Neural stem cell Neuron Cell differentiation Retinoic acid Nestin betaⅢ tubulin Immunohistochemistry Rat
ISSN号	0529-1356
其他题名	大鼠胚胎神经干细胞与永生化神经干细胞系C17.2定向分化为神经元潜能的差异
文献子类	Article
英文摘要	Objective To compare directed differentiation potential into neurons between neural stem cells(NSCs) from early embryonic rats and immortalized neural stem cell line C17.2(C17.2-NSC), and to establish a screening assay on the level of NSCs for high content screening in order to discover drugs that induce directed neurons differentiation of NSCs. Methods Three types of cells, the C17.2-NSC, 17 days embryonic hippocampus NSCs and 11 days embryonic cerebral cortex NSCs were used in this study. Cells from each type were divided into the control and experimental groups, and induced to differentiate by 0 mumol/L and 1 mumol/L retinoic acid (RA) for 5 days under DMEM/F12 medium containing 2% (V/V) B27 and 37℃, 5% C02 normal culture condition. The variability of expression level of nestin and beta Ⅲ tubulin (Tujl), which are the specific markers of neural stem and progenitor cells and neurons, respectively, in both control group and experimental group were detected through immunohistochemistry. Results Compared with the control group, C17.2-NSC did not differentiate into neurons under the induction of 1 mumol/L RA, but 17-day embryonic hippocampus NSCs and 11-day embryonic cerebral cortex NSCs effectively differentiated into neurons under the same treatment. Conclusion The directed differentiation capacity of early embryonic NSCs into neurons is higher than that of C17.2-NSC, thus early embryonic NSCs are suitable for high content drug screening which aims to discover new types of compounds that induce directed neurons differentiation of NSCs.
资助项目	国家自然科学基金资助项目[00000000]
WOS研究方向	General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:4696544
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267813]
专题	国家新药筛选中心
作者单位	1.National Center for Drug Screening, Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. 2.School of Life Sciences, East China Normal University, Shanghai 200062, China.;
推荐引用方式 GB/T 7714	Xie Junling,Zhang Yue,Huang Xiaojie,et al. Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2[J]. Acta Anatomica Sinica,2012,43(5):581-587.
APA	Xie Junling,Zhang Yue,Huang Xiaojie,&Zang Yi.(2012).Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2.Acta Anatomica Sinica,43(5),581-587.
MLA	Xie Junling,et al."Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2".Acta Anatomica Sinica 43.5(2012):581-587.