Development of a high-throughput screening assay for metabolic syndrome | |
Chen DongMei1; Qiu BeiYing2; Yang LingLing1; Li JingYa2![]() | |
刊名 | Chinese Science Bulletin
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2013 | |
卷号 | 58期号:4页码:321-328 |
关键词 | L6 myotube mitochondrial membrane potential high-throughput screening metabolic syndrome |
ISSN号 | 0023-074X |
其他题名 | 代谢综合征药物高通量筛选模型的建立 |
文献子类 | Article |
英文摘要 | Mitochondria are one of the most important organelles in eukaryotic cells. In addition to supplying energy to cells, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation and growth, and cell death. They have been implicated in several human diseases, including diabetes, obesity, neurodegenerative diseases and cancer. Mitochondrial membrane potential provides an index of mitochondrial functions. We developed a high-throughput screening assay for mitochondrial membrane potential in metabolic L6 myotubes. We optimized the concentration and processing time for JC-1 dye, the processing time for compounds, and the concentration of the uncoupler carbonylcyanide-m-chlorophenylhydrazone (CCCP). The screening assay was verified with the mitochondrial inhibitors rotenone, malonate, antimycin A, oligomycin and berberine. The coefficient of variation (CV) with 10 mumol L~(?1) CCCP was 5.92%, and the Z′ factor was 0.575; thereby meeting the strict quality control criteria for high-throughput screening assays. Our results indicate that L6 myotubes are appropriate, sensitive and efficient for measuring mitochondrial function, and could increase the likelihood of finding novel lead compounds for the treatment of metabolic syndrome. |
资助项目 | 国家自然科学基金[00000000] ; 中国科学院创新药物研发网络[00000000] ; 上海市科委公共服务平台项目[00000000] |
WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
语种 | 中文 |
CSCD记录号 | CSCD:4773216 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/267794] ![]() |
专题 | 国家新药筛选中心 |
作者单位 | 1.School of Life Sciences,East China Normal University, Shanghai 200062, China.; 2.National Center for Drug Screening,State Key Laboratory of New Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
推荐引用方式 GB/T 7714 | Chen DongMei,Qiu BeiYing,Yang LingLing,et al. Development of a high-throughput screening assay for metabolic syndrome[J]. Chinese Science Bulletin,2013,58(4):321-328. |
APA | Chen DongMei,Qiu BeiYing,Yang LingLing,&Li JingYa.(2013).Development of a high-throughput screening assay for metabolic syndrome.Chinese Science Bulletin,58(4),321-328. |
MLA | Chen DongMei,et al."Development of a high-throughput screening assay for metabolic syndrome".Chinese Science Bulletin 58.4(2013):321-328. |
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