Development of a high-throughput screening assay for metabolic syndrome

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	Development of a high-throughput screening assay for metabolic syndrome
	Chen DongMei 1; Qiu BeiYing 2; Yang LingLing 1; Li JingYa2
刊名	Chinese Science Bulletin
	2013
卷号	58 期号:4 页码:321-328
关键词	L6 myotube mitochondrial membrane potential high-throughput screening metabolic syndrome
ISSN号	0023-074X
其他题名	代谢综合征药物高通量筛选模型的建立
文献子类	Article
英文摘要	Mitochondria are one of the most important organelles in eukaryotic cells. In addition to supplying energy to cells, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation and growth, and cell death. They have been implicated in several human diseases, including diabetes, obesity, neurodegenerative diseases and cancer. Mitochondrial membrane potential provides an index of mitochondrial functions. We developed a high-throughput screening assay for mitochondrial membrane potential in metabolic L6 myotubes. We optimized the concentration and processing time for JC-1 dye, the processing time for compounds, and the concentration of the uncoupler carbonylcyanide-m-chlorophenylhydrazone (CCCP). The screening assay was verified with the mitochondrial inhibitors rotenone, malonate, antimycin A, oligomycin and berberine. The coefficient of variation (CV) with 10 mumol L~(?1) CCCP was 5.92%, and the Z′ factor was 0.575; thereby meeting the strict quality control criteria for high-throughput screening assays. Our results indicate that L6 myotubes are appropriate, sensitive and efficient for measuring mitochondrial function, and could increase the likelihood of finding novel lead compounds for the treatment of metabolic syndrome.
资助项目	国家自然科学基金[00000000] ; 中国科学院创新药物研发网络[00000000] ; 上海市科委公共服务平台项目[00000000]
WOS研究方向	General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:4773216
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267794]
专题	国家新药筛选中心
作者单位	1.School of Life Sciences,East China Normal University, Shanghai 200062, China.; 2.National Center for Drug Screening,State Key Laboratory of New Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	Chen DongMei,Qiu BeiYing,Yang LingLing,et al. Development of a high-throughput screening assay for metabolic syndrome[J]. Chinese Science Bulletin,2013,58(4):321-328.
APA	Chen DongMei,Qiu BeiYing,Yang LingLing,&Li JingYa.(2013).Development of a high-throughput screening assay for metabolic syndrome.Chinese Science Bulletin,58(4),321-328.
MLA	Chen DongMei,et al."Development of a high-throughput screening assay for metabolic syndrome".Chinese Science Bulletin 58.4(2013):321-328.