Effects of glucose and insulin on expression and function of bile acid transporters in sandwich cultured rat hepatocytes

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	Effects of glucose and insulin on expression and function of bile acid transporters in sandwich cultured rat hepatocytes
	He Lei 2; Guo Cen 2; Yao Dan 1; Sheng Jingjing 1; Pan Guoyu1 ; Liu Xiaodong 2
刊名	Chinese Journal of Clinical Pharmacology and Therapeutics
	2013
卷号	18 期号:8 页码:847-852
关键词	Glucose Insulin Sandwich cultured rat hepatocyte Bile acid transporter
ISSN号	1009-2501
其他题名	葡萄糖和胰岛素调节三明治肝细胞中胆酸相关转运体表达及功能
文献子类	Article
英文摘要	AIM:To explain the enhancement of bile acid excretion in diabetes from bile acid transporter perspective. METHODS:Primary rat hepatocytes were isolated and cultured in a sandwich configuration(SCRH).Then hepatocytes were cultured with different concentrations of glucose and insulin,and function of Na~+/bile acid cotransporter (ntcp)and bile salt export pump(bsep)were tested with Cholyl-lysyl-fluorescein (CLF)as a typical substrate.mRNA levels of ntcp,bsep and lipid regulating nuclear receptor fxr were determined with Real Time PCR.RESULTS:CLF biliary excretion was both decreased in high concentrations of glucose and insulin significantly.Both high concentrations of glucose and insulin can decrease ntcp mRNA for 3-4fold,but increase bsep mRNA for approximately 2fold.Levels of fxr mRNA was down regulated in high concentrations of glucose and up regulated in high concentrations of insulin. CONCLUSION:Abnormal glucose and insulin concentration have impacts on bile acid transporters in sandwich cultured rat hepatocytes. Sandwich cultured rat hepatocytes could offer a useful tool to explore hepatobiliary disposition of bile acid and drugs.
资助项目	国家自然科学基金面上项目新型体外模型研究糖尿病中肝脏胆酸药物转运体功能变化[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:4921305
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267759]
专题	药物安全性评价中心
通讯作者	Pan Guoyu
作者单位	1.Shanghai Institute of Materia Medica,Center for Drug Safety Evaluation and Research, Shanghai 201203, China. 2.China Pharmaceutical University,Drug Metabolism and Pharmacokinetics, Nanjing, Jiangsu 210009, China.;
推荐引用方式 GB/T 7714	He Lei,Guo Cen,Yao Dan,et al. Effects of glucose and insulin on expression and function of bile acid transporters in sandwich cultured rat hepatocytes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics,2013,18(8):847-852.
APA	He Lei,Guo Cen,Yao Dan,Sheng Jingjing,Pan Guoyu,&Liu Xiaodong.(2013).Effects of glucose and insulin on expression and function of bile acid transporters in sandwich cultured rat hepatocytes.Chinese Journal of Clinical Pharmacology and Therapeutics,18(8),847-852.
MLA	He Lei,et al."Effects of glucose and insulin on expression and function of bile acid transporters in sandwich cultured rat hepatocytes".Chinese Journal of Clinical Pharmacology and Therapeutics 18.8(2013):847-852.