γ-Secretase Epsilon-cleavage Assay

doi:10.21769/BioProtoc.2900

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	γ-Secretase Epsilon-cleavage Assay
	Xu, Ting-Hai 3; Yan, Yan 3; Harikumar, Kaleeckal G 1; Miller, Laurence J 1; Melcher, Karsten 2; Xu, H Eric 3
刊名	Bio-protocol
	2017-11-20
卷号	7 期号:22
ISSN号	2331-8325
DOI	10.21769/BioProtoc.2900
文献子类	Article
英文摘要	γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang ., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are HEK293 cells with a stably integrated luciferase reporter under the control of the bacterial tetO operator element, in which C99 C terminally fused to a reversed tetracyclin-inducible activator (rTA) transcriptional activator is expressed. Endogenous or transfected γ-secretase cleaves a C terminally fused rTA transcriptional activator from C99, allowing rTA to move to the nucleus to activate a luciferase reporter gene as a measurement for γ-secretase cleavage activity.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266296]
专题	药物靶标结构与功能中心
作者单位	1.Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA; 2.Center for Cancer and Cell Biology, Innovation and Integration Program, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA 3.Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
推荐引用方式 GB/T 7714	Xu, Ting-Hai,Yan, Yan,Harikumar, Kaleeckal G,et al. γ-Secretase Epsilon-cleavage Assay[J]. Bio-protocol,2017,7(22).
APA	Xu, Ting-Hai,Yan, Yan,Harikumar, Kaleeckal G,Miller, Laurence J,Melcher, Karsten,&Xu, H Eric.(2017).γ-Secretase Epsilon-cleavage Assay.Bio-protocol,7(22).
MLA	Xu, Ting-Hai,et al."γ-Secretase Epsilon-cleavage Assay".Bio-protocol 7.22(2017).