| Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization | |
| Xu, Ting-Hai1; Yan, Yan1; Harikumar, Kaleeckal G3; Miller, Laurence J3; Melcher, Karsten2; Xu, H Eric1 | |
| 刊名 | Bio-protocol
 |
| 2017-11-20 | |
| 卷号 | 7期号:22 |
| ISSN号 | 2331-8325 |
| DOI | 10.21769/BioProtoc.2901 |
| 文献子类 | Article |
| 英文摘要 | Pulldown assay is a conventional method to determine protein-protein interactions . Expressing a protein of interest with two different tags allows testing whether both versions can be captured via one of the two tags as homooligomeric complex. This protocol is based on streptavidin bead capture of a biotinylated protein and co-associated Flag-tagged protein using Streptavidin MagBeads. |
| 语种 | 英语 |
| 内容类型 | 期刊论文 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266288]  |
| 专题 | 药物靶标结构与功能中心 |
| 作者单位 | 1.Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 2.Center for Cancer and Cell Biology, Innovation and Integration Program, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA 3.Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA; |
| 推荐引用方式 GB/T 7714 | Xu, Ting-Hai,Yan, Yan,Harikumar, Kaleeckal G,et al. Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization[J]. Bio-protocol,2017,7(22). |
| APA | Xu, Ting-Hai,Yan, Yan,Harikumar, Kaleeckal G,Miller, Laurence J,Melcher, Karsten,&Xu, H Eric.(2017).Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization.Bio-protocol,7(22). |
| MLA | Xu, Ting-Hai,et al."Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization".Bio-protocol 7.22(2017). |
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