Investigation of the immunosuppressive activity of Physalin H on T lymphocytes

doi:10.1016/j.intimp.2009.11.013

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Investigation of the immunosuppressive activity of Physalin H on T lymphocytes
	Yu, Youjun 2; Sun, Lijuan 2; Ma, Lei 2; Li, Jiyu 3; Hu, Lihong 1,2; Liu, Jianwen 2
刊名	INTERNATIONAL IMMUNOPHARMACOLOGY
	2010-03
卷号	10 期号:3 页码:290-297
关键词	Physalin H Immunosuppressive Cell mitosis cycle Delayed-type hypersensitivity Th1/Th2 balance Heme oxygenase-1
ISSN号	1567-5769
DOI	10.1016/j.intimp.2009.11.013
文献子类	Article
英文摘要	Physalis angulata is an annual herb widely used in folk medicine. It is mainly used for treating rheumatoid arthritis (RA). Following bioactivity-guided isolation, a representative immunosuppressive compound, Physalin H was been identified from this herb medicine. The purpose of this work was to assess the immunosuppressive activity of Physalin H on T cells and to explore its potential mode of action. The results showed that Physalin H in a dose-dependent manner significantly inhibited the proliferation of T cells induced by concanavalin A (ConA) and by the mixed lymphocyte culture reaction (MLR). This inhibitive activity was mainly due to interfering DNA replication in G1 stages. In vivo experiments showed that, administration of Physalin H dose-dependently suppressed CD4(+) T cell mediated delayed-type hypersensitivity (DTH) reactions, and suppressed antigen-specific T-cell response in ovalbumin (OVA) immunized mice. Further study indicated that Physalin H could modulate Th1/Th2 cytokine balance and induce the production of immune regulation target Heme oxygenase (HO)-1 in T-cells in vitro. In this study, we demonstrated the immunosuppressive effect of Physalin H on T cells both in vitro and in vivo, and the immunosuppressive activity might be attributed to the suppression of T cell activation and proliferation, the modulation of Th1/Th2 cytokine balance and the induction of HO-1 in T cells. (C) 2009 Elsevier B.V. All rights reserved.
资助项目	National Special Fund for State Key Laboratory of Bioreactor Engineering[2060204] ; 111 Project[B07023] ; Shanghai Leading Academic Discipline Project[B507] ; National Nature Science Foundation of China-Project for Young Scientists Fund[30600598] ; Project of Shanghai Science Committee for Young Scientists[06QA14034]
WOS关键词	HEME OXYGENASE-1 ; AUTOIMMUNE-DISEASES ; CELL-ACTIVATION ; IMMUNE-RESPONSE ; RAT-BRAIN ; IN-VITRO ; INDUCTION ; EXPRESSION ; OVEREXPRESSION ; MECHANISMS
WOS研究方向	Immunology ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000275925700005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278966]
专题	上海中药现代化研究中心
通讯作者	Li, Jiyu
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Gen Surg, Shanghai 200092, Peoples R China
推荐引用方式 GB/T 7714	Yu, Youjun,Sun, Lijuan,Ma, Lei,et al. Investigation of the immunosuppressive activity of Physalin H on T lymphocytes[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2010,10(3):290-297.
APA	Yu, Youjun,Sun, Lijuan,Ma, Lei,Li, Jiyu,Hu, Lihong,&Liu, Jianwen.(2010).Investigation of the immunosuppressive activity of Physalin H on T lymphocytes.INTERNATIONAL IMMUNOPHARMACOLOGY,10(3),290-297.
MLA	Yu, Youjun,et al."Investigation of the immunosuppressive activity of Physalin H on T lymphocytes".INTERNATIONAL IMMUNOPHARMACOLOGY 10.3(2010):290-297.