Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry

doi:10.1002/cbdv.200900387

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	Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry
	Ye, Guan 2; Tang, Yi-Hong 2; Wang, Gui-Yun 1; Li, Zhi-Xiong2 ; Zhu, Hai-Yan 2; Ma, Chun-Hui 2; Sun, Zhao-Ling 2; Huang, Cheng-Gang2
刊名	CHEMISTRY & BIODIVERSITY
	2010-12
卷号	7 期号:12 页码:2917-2930
ISSN号	1612-1872
DOI	10.1002/cbdv.200900387
文献子类	Article
英文摘要	A rapid, sensitive, and specific method by high-performance liquid chromatography (HPLC) coupled to diode-array detection (DAD) and tandem mass spectrometry (MS) techniques was developed for the identification of absorbed constituents and their metabolites in rats after the oral administration of a Chai-Huang decoction (CHD), which consists of Bupleurum chinense and Scutellaria baicalensis in the proportion 1:1 (w/w). By comparing their retention times and MS data with those of authentic compounds and published data, a total of 14 compounds were identified in the CHD samples. In addition, eleven and seven compounds were characterized in the urine and serum samples of the rats, respectively. The results indicated that the main absorbed constituents were chrysin-6-C-arabinosyl-8-C-glucoside, chrysin-6-C-glucosyl-8-C-arabinoside, baicalin, wogonin 5-O-glucoside, oroxylin A-7-O-glucuronide, wogonoside, saikosaponin A, saikosaponin C, saikosaponin D, baicalein, and wogonin. These compounds might be responsible for the curative effects of the CHD. The findings demonstrated that the proposed method could be used to rapidly and simultaneously analyze and screen the multiple absorbed bioactive constituents in a formula of traditional Chinese medicines (TCM). This is very important not only for the pharmaceutical discovery process and the quality control of crude drugs but also to explain the mechanisms of action of TCM.
资助项目	Science and Technology Commission of the Shanghai Municipality[02DZ19145] ; National Science & Technology Major Project[2009ZX09301-001]
WOS关键词	SCUTELLARIA-BAICALENSIS-GEORGI ; LC-MS/MS ; MAJOR METABOLITES ; MEDICINAL HERBS ; RADIX-BUPLEURI ; BAICALIN ; IDENTIFICATION ; WOGONIN ; EXTRACT ; URINE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000285393800011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278702]
专题	上海中药现代化研究中心
通讯作者	Huang, Cheng-Gang
作者单位	1.Anshan Normal Univ, Anshan Hlth Sch, Anshan 114001, Liaoning Prov, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Ye, Guan,Tang, Yi-Hong,Wang, Gui-Yun,et al. Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry[J]. CHEMISTRY & BIODIVERSITY,2010,7(12):2917-2930.
APA	Ye, Guan.,Tang, Yi-Hong.,Wang, Gui-Yun.,Li, Zhi-Xiong.,Zhu, Hai-Yan.,...&Huang, Cheng-Gang.(2010).Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry.CHEMISTRY & BIODIVERSITY,7(12),2917-2930.
MLA	Ye, Guan,et al."Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry".CHEMISTRY & BIODIVERSITY 7.12(2010):2917-2930.