Clarifying the signal network of salvianolic acid B using proteomic assay and bioinformatic analysis

doi:10.1002/pmic.201000482

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	Clarifying the signal network of salvianolic acid B using proteomic assay and bioinformatic analysis
	Feng, Li-Xing 1; Jing, Chun-Jie 1; Tang, Kai-Lin 2; Tao, Lin 2; Cao, Zhi-Wei 2; Wu, Wan-Ying1 ; Guan, Shu-Hong1 ; Jiang, Bao-Hong1 ; Yang, Min 1; Liu, Xuan 1
刊名	PROTEOMICS
	2011-04
卷号	11 期号:8 页码:1473-1485
关键词	2-DE H9C2 cell Bioinformatics Epidermal growth factor receptor Salvianolic acid B
ISSN号	1615-9853
DOI	10.1002/pmic.201000482
文献子类	Article
英文摘要	Salvianolic acid B (SB) is a natural compound with protective effect against ischemia-reperfusion heart injury. However, the signal network of SB including both direct target proteins and downstream signal-related proteins has not been clarified. In the present study, epidermal growth factor receptor (EGFR) was predicted to be the most possible direct protein target of SB by INVDOCK, a ligand-protein inverse-docking algorithm. Possible signal-related proteins of SB in H9C2 cells, including both under normal condition and under ischemia-reperfusion injury, were searched using 2-DE analysis. Totally, 14 signal-related proteins were found. Finally, signal network from EGFR to the signal-related proteins was established using bioinformatic analysis. Interestingly, 9 of the 14 signal-related proteins could be included in a network together with EGFR through direct interaction or only one intermediate partner. The signal cascade from EGFR to heat shock protein 27 (HSP27) and mitofilin (IMMT, inner membrane mitochondrial protein) might be the most important cascade. The signal network was certified by measuring the binding affinity of SB to EGFR in vitro, the effect of SB on internalization and phosphorylation of EGFR, the effect of SB on viability and proliferation of H9C2 cells, and the expression of inner membrane mitochondrial protein in the presence of EGFR inhibitor AG 1478.
WOS关键词	MOLECULAR INTERACTION DATABASE ; EPIDERMAL-GROWTH-FACTOR ; MYOCARDIAL-INFARCTION ; COILED-COIL ; PROTEIN ; MILTIORRHIZA ; MITOFILIN ; SEARCH ; ISCHEMIA ; UPDATE
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000289528800009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278568]
专题	上海中药现代化研究中心
通讯作者	Guo, De-An
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; 2.Tongji Univ, Coll Life Sci & Technol, Shanghai 200092, Peoples R China
推荐引用方式 GB/T 7714	Feng, Li-Xing,Jing, Chun-Jie,Tang, Kai-Lin,et al. Clarifying the signal network of salvianolic acid B using proteomic assay and bioinformatic analysis[J]. PROTEOMICS,2011,11(8):1473-1485.
APA	Feng, Li-Xing.,Jing, Chun-Jie.,Tang, Kai-Lin.,Tao, Lin.,Cao, Zhi-Wei.,...&Guo, De-An.(2011).Clarifying the signal network of salvianolic acid B using proteomic assay and bioinformatic analysis.PROTEOMICS,11(8),1473-1485.
MLA	Feng, Li-Xing,et al."Clarifying the signal network of salvianolic acid B using proteomic assay and bioinformatic analysis".PROTEOMICS 11.8(2011):1473-1485.