Quality assessment for fructus Gardeniae by multi-component quantification, chromatographic fingerprint and related chemometric analysis

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	Quality assessment for fructus Gardeniae by multi-component quantification, chromatographic fingerprint and related chemometric analysis
	Yang, Quanjun 1,2; Fan, Mingsong 2; Wu, Bin 2; Sun, Zhaolin 2; Cui, Xiaolan 3; Du, Xiaowei 1; Huang, Chenggang2
刊名	JOURNAL OF MEDICINAL PLANTS RESEARCH
	2011-09-09
卷号	5 期号:17 页码:4126-4137
关键词	Fructus Gardeniae chromatographic fingerprint multi-component quantification principal component analysis hierachical cluster analysis
ISSN号	1996-0875
文献子类	Article
英文摘要	A simple, feasible and effective method of ultra performance liquid chromatography (UPLC) coupled with photo diode array (PDA) were established for fingerprint analysis and simultaneous quantification of three major classes of constitutions including iridoid glycosides, crocins and organic acids of fructus Gardeniae. Extraction method was optimized as 75% methanol ultrasonic extraction for 30 min. Acetonitrile-water (containing 0.2% formic acid) gradient elution on Waters Acquity BEH C18 column (50 x 2.1 mm, 1.7 mu m) was used to obtain good chromatographic resolution. 24 characteristic peaks were comprised in the fingerprint common pattern. Among them, seven marked components, geniposide, shanzhiside methyl ester, geinpin, geniposidic acid, crocin I, crocin II, and chlorogenic acid, were quantified. Similarity evaluation, principal component analysis and hierachical cluster analysis were applied to demonstrate the distinction. It was concluded that chemical components of Gardenia jasminoides Eills and Gardenia jasminoides var. radicans Makino from different origins were similar. Other than determination of the content of geniposide and crocin I, comparison of fingerprint atlas could be considered as a suitable quality control method for fructus Gardeniae.
资助项目	China National Key Hi-Tech Innovation Project for the R&D of Novel Drugs[2009ZX09301-001] ; China National Key Hi-Tech Innovation Project for the R&D of Novel Drugs[2009ZX09308-005] ; China National Key Hi-Tech Innovation Project for the R&D of Novel Drugs[2009ZX09103-373]
WOS关键词	TRADITIONAL CHINESE MEDICINES ; MASS-SPECTROMETRY ; JASMINOIDES ; GENIPOSIDE ; CROCIN ; FRUIT ; HPLC
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ACADEMIC JOURNALS
WOS记录号	WOS:000297465300020
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278404]
专题	上海中药现代化研究中心
通讯作者	Du, Xiaowei
作者单位	1.Heilongjiang Univ Chinese Med, Coll Pharm, Harbin 150040, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.China Acad Chinese Med Sci, Inst Chinese Med, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Yang, Quanjun,Fan, Mingsong,Wu, Bin,et al. Quality assessment for fructus Gardeniae by multi-component quantification, chromatographic fingerprint and related chemometric analysis[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH,2011,5(17):4126-4137.
APA	Yang, Quanjun.,Fan, Mingsong.,Wu, Bin.,Sun, Zhaolin.,Cui, Xiaolan.,...&Huang, Chenggang.(2011).Quality assessment for fructus Gardeniae by multi-component quantification, chromatographic fingerprint and related chemometric analysis.JOURNAL OF MEDICINAL PLANTS RESEARCH,5(17),4126-4137.
MLA	Yang, Quanjun,et al."Quality assessment for fructus Gardeniae by multi-component quantification, chromatographic fingerprint and related chemometric analysis".JOURNAL OF MEDICINAL PLANTS RESEARCH 5.17(2011):4126-4137.