Matrix metalloproteinase-9 induces cardiac fibroblast migration, collagen and cytokine secretion: Inhibition by salvianolic acid B from Salvia miltiorrhiza

doi:10.1016/j.phymed.2011.06.024

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	Matrix metalloproteinase-9 induces cardiac fibroblast migration, collagen and cytokine secretion: Inhibition by salvianolic acid B from Salvia miltiorrhiza
	Wang, Yanhui 1,2; Xu, Feng 2; Chen, Jing1 ; Shen, Xu1 ; Deng, Yanping 1; Xu, Lingling 1; Yin, Jun 2; Chen, Hui 1,2; Teng, Fukang 1,2; Liu, Xuan 1
刊名	PHYTOMEDICINE
	2011-12-15
卷号	19 期号:1 页码:13-19
关键词	Matrix metalloproteinases-9 Salvianolic acid B Salvia miltiorrhiza Cardiac fibroblast Migration Cytokine
ISSN号	0944-7113
DOI	10.1016/j.phymed.2011.06.024
文献子类	Article
英文摘要	Cardiac fibroblasts play the key role in cardiac function and matrix metalloproteinases-9 (MMP-9) is a well known contributor to the development of myocardial remodeling. However, the direct regulation of MMP-9 on the function of cardiac fibroblasts and the underlying mechanism are far from elucidation. In the present research, recombinant protein encoding catalytic domain of MMP-9 (MMP-9 CD) was constructed and the function of neonatal cardiac fibroblasts was investigated by cell proliferation assay, migration assay, picrosirius red assay, multiplex cytokine assay and fibroblast phenotype detection. 200 nM MMP-9 CD stimulated cardiac fibroblasts migration (169.4 +/- 22.5% versus 100 +/- 0%. p<0.01), increased collagen synthesis (1.5 +/- 0.2 fold, p<0.05), up-regulated the secretion of ICAM (574.0 +/- 40.1 versus 268.5 +/- 8.6 pg/ml, p<0.01), INF-alpha (192.6 +/- 11.0 versus 14.4 +/- 1.8 pg/ml, p<0.001), 1L-6 (1500.9 +/- 70.2 versus 323.4 +/- 40.6 pg/ml, p<0.001) and sVCAM-1 (30.3 +/- 4.3 versus 7.0 +/- 0.1 pg/ml, p<0.05) and down-regulated VEGF (436.5 +/- 148.9 versus 1034.3 +/- 28.1 pg/ml, p<0.05) significantly with modest effects on proliferation. Accompanying with these regulations, transition of fibroblasts to myofibroblast was confirmed by immunofluorescent stain of a-smooth muscle actin (alpha-SMA) with MMP-9 CD treatment. Furthermore, salvianolic acid B (SalB) inhibited the effects of MMP-9 CD significantly. In conclusion, our results provide evidence for a direct influence of MMP-9 on cardiac fibroblast migration, collagen and cytokine secretion, which can be attenuated by SalB. (C) 2011 Elsevier GmbH. All rights reserved.
资助项目	National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2009ZX09308-005] ; National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2009ZX09102-122] ; National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2009ZX090304-002] ; National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2009ZX09311-001] ; National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2009ZX09502-020] ; Chinese Academy of Science[KSCX2-YW-R-166]
WOS关键词	MATRIX METALLOPROTEINASES ; MMP-9 ; CELL ; PROLIFERATION ; EXPRESSION ; INVASION ; FAILURE ; DOMAIN
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
WOS记录号	WOS:000299456900003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278301]
专题	上海中药现代化研究中心
通讯作者	Jiang, Baohong
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanhui,Xu, Feng,Chen, Jing,et al. Matrix metalloproteinase-9 induces cardiac fibroblast migration, collagen and cytokine secretion: Inhibition by salvianolic acid B from Salvia miltiorrhiza[J]. PHYTOMEDICINE,2011,19(1):13-19.
APA	Wang, Yanhui.,Xu, Feng.,Chen, Jing.,Shen, Xu.,Deng, Yanping.,...&Guo, De-an.(2011).Matrix metalloproteinase-9 induces cardiac fibroblast migration, collagen and cytokine secretion: Inhibition by salvianolic acid B from Salvia miltiorrhiza.PHYTOMEDICINE,19(1),13-19.
MLA	Wang, Yanhui,et al."Matrix metalloproteinase-9 induces cardiac fibroblast migration, collagen and cytokine secretion: Inhibition by salvianolic acid B from Salvia miltiorrhiza".PHYTOMEDICINE 19.1(2011):13-19.