Estrogenic effects of ginsenoside Rg1 in endometrial cells in vitro were not observed in immature CD-1 mice or ovariectomized mice model

doi:10.1097/gme.0b013e318250361c

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	Estrogenic effects of ginsenoside Rg1 in endometrial cells in vitro were not observed in immature CD-1 mice or ovariectomized mice model
	Chen, Wen-Fang 2,3; Gao, Quan-Gui 2; Dai, Zhi-Jie 4; Kung, Annie Wai-Chee 4; Guo, De-an1 ; Wong, Man-Sau 2,5
刊名	MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY
	2012-09
卷号	19 期号:9 页码:1052-1061
关键词	Ginsenoside Rg1 Estrogen receptor Ishikawa cells MC3T3-E1 cells CD-1 mice C57BL/6J mice
ISSN号	1072-3714
DOI	10.1097/gme.0b013e318250361c
文献子类	Article
英文摘要	Objective: The present study was designed to determine whether ginsenoside Rg1 could exert selective estrogenic effects by using both cell lines and an animal model. Methods: The endometrial Ishikawa cells and preosteoblastic MC3T3-E1 cells were treated with a different dose of Rg1. Immature CD-1 mice and ovariectomized (OVX) C57BL/6J mice were used to study the short-term and long-term estrogenic effects of Rg1, respectively. Results: Rg1 significantly increased estrogen receptor-dependent alkaline phosphatase activity, activated estrogen response elementYluciferase activity, and induced the phosphorylation of mitogen-activated protein kinase kinase, extracellular-regulated kinase, and estrogen receptor-alpha in Ishikawa cells. In contrast, Rg1 did not induce any estrogenic responses in MC3T3-E1 cells. Administration of Rg1 to immature CD-1 mice did not alter their uterine weight or the estrogen-regulated gene expressions in the uterus. Treatment of OVX C57BL/6J mice with Rg1 via mini-osmotic pumps for 3 months did not alter the uterine weight or induce any transcriptional activation of estrogen receptor in the uterus. Rg1 induced Bcl-2 messenger RNA expression in the left ventricular tissue and striatum but failed to alter the bone mineral density in the femur and tibia of the OVX mice. Conclusions: Rg1 exerted potent estrogenic effects in endometrial cells in vitro as well as in heart and brain tissues in vivo. However, it did not exert any estrogenic effects on reproductive tissues in vivo, nor did it stimulate bone tissues in vitro or in vivo. Our results suggest that the estrogenic effects of Rg1 are distinct from those of estradiol and are cell type and tissue selective.
资助项目	Areas of Excellence Scheme under University Grants Committee of the Hong Kong Special Administrative Region, China[AOE/P-10/01] ; Research Grants Council[POLYU 5636/07M] ; Research Committee of The Hong Kong Polytechnic University[00000000]
WOS关键词	LIGAND-INDEPENDENT ACTIVATION ; HORMONE REPLACEMENT THERAPY ; CANCER MCF-7 CELLS ; ER-ALPHA ; OSTEOBLASTIC DIFFERENTIATION ; RECEPTOR MODULATOR ; SIGNALING PATHWAY ; GENE-EXPRESSION ; ISHIKAWA CELLS ; MC3T3-E1 CELLS
WOS研究方向	Obstetrics & Gynecology
语种	英语
出版者	LIPPINCOTT WILLIAMS & WILKINS
WOS记录号	WOS:000308415500018
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277955]
专题	上海中药现代化研究中心
通讯作者	Wong, Man-Sau
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat, Shanghai 200031, Peoples R China; 2.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China; 3.Qingdao Univ, Coll Med, Dept Physiol, Qingdao 266071, Peoples R China; 4.Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China; 5.State Key Lab Chinese Med & Mol Pharmacol Incubat, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Chen, Wen-Fang,Gao, Quan-Gui,Dai, Zhi-Jie,et al. Estrogenic effects of ginsenoside Rg1 in endometrial cells in vitro were not observed in immature CD-1 mice or ovariectomized mice model[J]. MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY,2012,19(9):1052-1061.
APA	Chen, Wen-Fang,Gao, Quan-Gui,Dai, Zhi-Jie,Kung, Annie Wai-Chee,Guo, De-an,&Wong, Man-Sau.(2012).Estrogenic effects of ginsenoside Rg1 in endometrial cells in vitro were not observed in immature CD-1 mice or ovariectomized mice model.MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY,19(9),1052-1061.
MLA	Chen, Wen-Fang,et al."Estrogenic effects of ginsenoside Rg1 in endometrial cells in vitro were not observed in immature CD-1 mice or ovariectomized mice model".MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY 19.9(2012):1052-1061.