Identification of xanthones from Swertia punicea using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry

doi:10.1002/rcm.6419

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	Identification of xanthones from Swertia punicea using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry
	Du, Xin-Gang 1,2; Wang, Wei 3,4; Zhang, Qing-Ying 2; Cheng, Jun 5; Avula, Bharathi 6; Khan, Ikhlas A.6; Guo, De-An1
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2012-12-30
卷号	26 期号:24 页码:2913-2923
ISSN号	0951-4198
DOI	10.1002/rcm.6419
文献子类	Article
英文摘要	RATIONALE Swertia punicea Hemsl. (Gentianaceae) are used mainly for the treatment of acute bilious hepatitis, cholecystitis, fever, intoxification and jaundice in China, as a traditional Chinese folk medicine. Xanthones as the main chemical components of Swertia punicea have many possible pharmacological properties, such as hepatoprotective and anti-HIV. In order to obtain an overall picture of the xanthones of Swertia punicea, high-performance liquid chromatography diode-array detection/tandem mass spectrometry (HPLC/DAD-ESI-MSn) was applied to the structural characterization of xanthones in ethyl acetate and acetone extracts. METHODS The ESI-MS fragmentation behaviors of xanthones were investigated based on the 17 reference xanthones and then applied to the structural characterization of xanthones in ethyl acetate and acetone extracts of Swertia punicea by HPLC/DAD-ESI-MSn. RESULTS The fragmentation rules of aglycone, C-glycosides, O-glycosides, and polyxanthones with different linkages were summarized. The observed fragmentation pathways were used successfully for the analysis of the xanthone constituents of Swertia punicea, and a total of 34 xanthones were identified, among which 16 compounds were new and one compound was reported from this species for the first time. CONCLUSIONS The described methods were very valuable for the identification of xanthones, especially of the trace compounds, and therefore could be utilized for sensitive and rapid qualitative analysis of xanthones in Swertia punicea. Copyright (C) 2012 John Wiley & Sons, Ltd.
资助项目	Program for Changjiang Scholar and Innovative Team in University[985-2-063-112] ; Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China[104218]
WOS关键词	JAPONICA MAKINO ; 3-O-DEMETHYLSWERTIPUNICOSIDE ; FRANCHETIANA ; GENTIANELLA ; GLUCOSIDES ; GLYCOSIDES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000310872500008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277836]
专题	上海中药现代化研究中心
通讯作者	Guo, De-An
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; 2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100191, Peoples R China; 3.Hunan Univ Chinese Med, Sch Pharm, TCM, Changsha 410208, Hunan, Peoples R China; 4.Hunan Univ Chinese Med, Sch Pharm, Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China; 5.China Agr Univ, Key Lab Urban Agr N, Minist Agr, Beijing 102206, Peoples R China; 6.Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA
推荐引用方式 GB/T 7714	Du, Xin-Gang,Wang, Wei,Zhang, Qing-Ying,et al. Identification of xanthones from Swertia punicea using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2012,26(24):2913-2923.
APA	Du, Xin-Gang.,Wang, Wei.,Zhang, Qing-Ying.,Cheng, Jun.,Avula, Bharathi.,...&Guo, De-An.(2012).Identification of xanthones from Swertia punicea using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,26(24),2913-2923.
MLA	Du, Xin-Gang,et al."Identification of xanthones from Swertia punicea using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 26.24(2012):2913-2923.