Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn

doi:10.1007/s10337-013-2475-x

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	Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn
	Li, Zhixiong1,2 ; Song, Xinmeng 2,3; Fu, Zhiwen 1,2; Wu, Bin 2; Ling, Yun 2; Sun, Zhaolin 2; Chen, Mingcang 2; Xu, Desheng 1; Huang, Chenggang2
刊名	CHROMATOGRAPHIA
	2013-07
卷号	76 期号:13-14 页码:767-780
关键词	LC-ESI-MSn Rhizoma Anemarrhenae and Radix Astragali Major constituents Metabolites
ISSN号	0009-5893
DOI	10.1007/s10337-013-2475-x
文献子类	Article
英文摘要	The Zhimu-Huangqi herb-pair is a famous Chinese herbal formula with a combination of Rhizoma Anemarrhenae (Zhimu in Chinese) and Radix Astragali (Huangqi in Chinese). This work describes a sensitive and specific LC-ES-MSn methodology for identification of the major constituents in Zhimu-Huangqi herb-pair extract and their metabolites in rats after oral administration. A total of 30 compounds have been identified or tentatively characterized from the herb-pair extract, and 13 of them were unambiguously identified by comparing the retention times and mass spectra with those of reference standards, while the other 17 compounds were tentatively identified on the basis of their MSn fragmentation behaviors and exact mass information from literature. Moreover, the metabolites in vivo were also identified. The Zhimu-Huangqi herb-pair extract was actively metabolized in rats, including four parent compounds and 8 metabolites in serum and seven parent compounds and 23 metabolites in urine. This study proposed a good example for the rapid identification of major constituents in complex systems such as herbal extract or traditional Chinese medicine formula, which facilitated the clarification of the metabolic pathway of the herbs in the body to better understand the action mechanism.
资助项目	State Key Program of the National Natural Science Foundation of China[81030065] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2012ZX09301001-001] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2013ZX09102027]
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ANEMARRHENA-ASPHODELOIDES RHIZOMES ; TRADITIONAL CHINESE MEDICINES ; STEROIDAL SAPONINS ; ASTRAGALUS-MEMBRANACEUS ; RADIX-ASTRAGALI ; IN-VIVO ; DECOCTION ; MONGHOLICUS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000320959000005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277565]
专题	上海中药现代化研究中心
通讯作者	Xu, Desheng
作者单位	1.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Harbin Univ Commerce, Harbin 150076, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhixiong,Song, Xinmeng,Fu, Zhiwen,et al. Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn[J]. CHROMATOGRAPHIA,2013,76(13-14):767-780.
APA	Li, Zhixiong.,Song, Xinmeng.,Fu, Zhiwen.,Wu, Bin.,Ling, Yun.,...&Huang, Chenggang.(2013).Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn.CHROMATOGRAPHIA,76(13-14),767-780.
MLA	Li, Zhixiong,et al."Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn".CHROMATOGRAPHIA 76.13-14(2013):767-780.