High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII

doi:10.1093/chromsci/bmt052

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII
	Jia, Yao 1,2; Wu, Bin 2; Fan, Mingsong 2; Wang, Jinhui 1; Huang, Jian 1; Huang, Chenggang2
刊名	JOURNAL OF CHROMATOGRAPHIC SCIENCE
	2014-05
卷号	52 期号:5 页码:418-422
ISSN号	0021-9665
DOI	10.1093/chromsci/bmt052
文献子类	Article
英文摘要	A sensitive method based on high-performance liquid chromatography-electrospray ionization tandem mass spectrometry was developed for the determination of timosaponin AIII (TA3) and its in vivo and in vitro metabolites. The rat plasma, urine, feces and tissue samples were collected after oral administration of TA3 at a single dose of 300 mg/kg. TA3 was incubated into artificial gastric juice and artificial intestinal juice. The in vivo and in vitro samples were purified by using liquid-liquid extraction. The structures of metabolites were elucidated by comparing their molecular weights, retention times and tandem mass spectrometric spectra with those of the parent drug. As a result, four metabolites (deglycosylated TA3, two hydroxylated TA3 and timosaponin BII) and the parent drug were found in in vivo and in vitro samples. In addition to the parent drug, one, one and two metabolites were identified in heart, urine and feces, respectively. Only the parent drug was detected in plasma, liver and kidney. One hydroxylation metabolite and TA3 were identified from incubation samples with AGJ, whereas two hydroxylation metabolites and TA3 were detected from the incubation with AIJ. This is the first systematic metabolism study of TA3. The biotransformation pathways of TA3 primarily included deglycosylation, hydroxylation and glycosylation.
资助项目	National Science and Technology Major Project Key New Drug Creation and Manufacturing Program of China[2012ZX09301001-001] ; National Natural Science Foundation of China[81030065] ; National Natural Science Foundation of China[81274055]
WOS关键词	RAT URINE ; ANEMARRHENA-ASPHODELOIDES ; STEROIDAL SAPONINS ; A-III ; IDENTIFICATION ; RHIZOMA ; MS/MS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000342734600008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277084]
专题	上海中药现代化研究中心
通讯作者	Huang, Jian
作者单位	1.Shenyang Pharmaceut Univ, Coll Chinese Tradit Mat Med, Shenyang, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Jia, Yao,Wu, Bin,Fan, Mingsong,et al. High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):418-422.
APA	Jia, Yao,Wu, Bin,Fan, Mingsong,Wang, Jinhui,Huang, Jian,&Huang, Chenggang.(2014).High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII.JOURNAL OF CHROMATOGRAPHIC SCIENCE,52(5),418-422.
MLA	Jia, Yao,et al."High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII".JOURNAL OF CHROMATOGRAPHIC SCIENCE 52.5(2014):418-422.