In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS

doi:10.1007/s10337-014-2681-1

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS
	Li, Dandan 1,2; Xue, Rui 1; Li, Zhixiong1 ; Chen, Mingcang 1; Jiang, Weixin 2; Huang, Chenggang1
刊名	CHROMATOGRAPHIA
	2014-06
卷号	77 期号:11-12 页码:853-858
关键词	HPLC-QTOF-MS/MS Timosaponin BIII Rat Metabolites
ISSN号	0009-5893
DOI	10.1007/s10337-014-2681-1
文献子类	Article
英文摘要	Timosaponin BIII, as one of the steroid saponins isolated from Anemarrhena asphodeloides Bge., was proved to have many pharmacological activities in recent years and became a natural active compound with good development prospect. In the present study, a simple and rapid method using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry was developed for the determination of the structures of timosaponin BIII and its metabolites in rats after administrating intragastrically at 300 mg kg(-1). By comparing their changes in molecular masses (Delta M), retention times and spectral patterns with those of the parent compound, nine metabolites were detected and identified in urine, and eight in plasma as well as four in brain. It is also indicated that the deglycosylation and oxidation reactions were the main metabolic pathways in the biotransformation of timosaponin BIII in vivo and the structures of the nine metabolites were identified and proposed to be timosaponin BII(M1), the hydroxylated metabolite of TBII(M2), the hydroxylated metabolites of TBIII(M3 and M4), deglycosylation and monooxygenation product of TBIII(M5), the deglycosylation product of TBII(M6), timosaponin AIII(M8), the isomers of timosaponin AIII(M7 and M9).
资助项目	National Natural Science Foundation of China[81030065] ; National Natural Science Foundation of China[81274055]
WOS关键词	ANEMARRHENA-ASPHODELOIDES ; STEROIDAL SAPONINS ; MASS-SPECTROMETRY ; PROFILE ; RHIZOMA ; URINE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000337158600012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277049]
专题	上海中药现代化研究中心
通讯作者	Huang, Chenggang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Media, Shanghai 201203, Peoples R China; 2.Harbin Univ Commerce, Harbin 150076, Peoples R China
推荐引用方式 GB/T 7714	Li, Dandan,Xue, Rui,Li, Zhixiong,et al. In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS[J]. CHROMATOGRAPHIA,2014,77(11-12):853-858.
APA	Li, Dandan,Xue, Rui,Li, Zhixiong,Chen, Mingcang,Jiang, Weixin,&Huang, Chenggang.(2014).In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS.CHROMATOGRAPHIA,77(11-12),853-858.
MLA	Li, Dandan,et al."In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS".CHROMATOGRAPHIA 77.11-12(2014):853-858.