New triterpenic acids from Uncaria rhynchophylla: Chemistry, NO-inhibitory activity, and tandem mass spectrometric analysis

doi:10.1016/j.fitote.2014.04.004

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	New triterpenic acids from Uncaria rhynchophylla: Chemistry, NO-inhibitory activity, and tandem mass spectrometric analysis
	Zhang, Yi-bei; Yang, Wen-zhi; Yao, Chang-liang; Feng, Rui-hong; Yang, Min; Guo, De-an; Wu, Wan-ying
刊名	FITOTERAPIA
	2014-07
卷号	96 页码:39-47
关键词	Uncaria rhynchophylla Uncarinic acid NO-inhibitory effect Tandem mass spectrometric fragmentation
ISSN号	0367-326X
DOI	10.1016/j.fitote.2014.04.004
文献子类	Article
英文摘要	Five new oleanane and ursane type triterpenes, namely uncarinic acids F-J (1-5), together with six known triterpenic acids (6-11) were isolated from the stems and hooks of Uncaria rhynchophylla. Structure elucidation of 1-5 was based on the integrated analyses of high-resolution MS data, 1D (H-1 NMR,C-13 NMR, DEPT) and 2D (HSQC, HMBC, ROESY) NMR spectra. Compounds 4, 10, and 11 exhibited weak inhibitory effects on LPS-induced NO production in RAW264.7 cells (with IC50 1.48, 7.01, and 1.89 mu M, respectively) with dexamethasone (IC50 0.04 mu M) and quercetin (IC50 0.86 mu M) as the positive controls. 19-OH substituted oleanane triterpenic acids (1, 2, 5, 8) were prone to eliminate CH2O3, whereas those ursane-type encompassing 19-OH (3, 6, 7, 9, 4) were featured by preferred cleavage of H2O while performing the negative collision-induced MS/MS fragmentation on an LTQ/Orbitrap mass spectrometer. (C) 2014 Elsevier B.V. All rights reserved.
资助项目	National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; Traditional Chinese Medicine Industry Research Special Project[201307002] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing program of the People's Republic of China[2011ZX09307-002-03]
WOS关键词	NITRIC-OXIDE ; CELLS ; TOMENTOSA ; PLANT
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000338979200006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277001]
专题	上海中药现代化研究中心
通讯作者	Wu, Wan-ying
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yi-bei,Yang, Wen-zhi,Yao, Chang-liang,et al. New triterpenic acids from Uncaria rhynchophylla: Chemistry, NO-inhibitory activity, and tandem mass spectrometric analysis[J]. FITOTERAPIA,2014,96:39-47.
APA	Zhang, Yi-bei.,Yang, Wen-zhi.,Yao, Chang-liang.,Feng, Rui-hong.,Yang, Min.,...&Wu, Wan-ying.(2014).New triterpenic acids from Uncaria rhynchophylla: Chemistry, NO-inhibitory activity, and tandem mass spectrometric analysis.FITOTERAPIA,96,39-47.
MLA	Zhang, Yi-bei,et al."New triterpenic acids from Uncaria rhynchophylla: Chemistry, NO-inhibitory activity, and tandem mass spectrometric analysis".FITOTERAPIA 96(2014):39-47.