Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses

doi:10.3724/SP.J.1009.2015.00232

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses
	Wei Shan-Shan 1; Yang Min 2; Chen Xin 2; Wang Qiu-Rong 2; Cui Ya-Jun 1
刊名	CHINESE JOURNAL OF NATURAL MEDICINES
	2015-03
卷号	13 期号:3 页码:232-240
关键词	Licorice Flavonoids Glycyrrhizic acid Simultaneous determination HPLC-DAD Orbitrap mass spectrometry
ISSN号	2095-6975
DOI	10.3724/SP.J.1009.2015.00232
文献子类	Article
英文摘要	To determine 13 flavonoids and glycyrrhizic acid in licorice (Glycyrrhiza spp.), several samples from different areas were examined by HPLC-DAD analysisThe analysis was performed on a Zorbax Extend-C-18 (250 mm x 4.6 mm, 5 mu m) column connected with a Zorbax Extend guard column (20 mm x 4.6 mm, 5 mu m). The mobile phase consisted of (A) acetonitrile and (B) 0.026% aqueous H3PO4 (V/V) using a gradient elution of 20%-25% A at 0-20 min, 25%-33% A at 20-30 min, 33%-50% A at 30-55 min, 50%-60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 urn. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 mu g.mL(-1), respectively. The relative recovery rates for the 14 compounds were in the range of 93.90%-106.73% with RSDs being less than 5%. Coefficient variations for intra-day and inter-day precisions were in the range of 0.27%-2.38% and 0.31%-3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions.
资助项目	National Natural Science Foundation of China[81001630] ; Natural Science Foundation of Shanghai[10ZR1436400] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06]
WOS关键词	CANCER CELLS ; URALENSIS ; LIQUIRITIN ; CHROMATOGRAPHY ; CONSTITUENTS ; EXTRACT ; GLABRA ; ROOT ; ISOLIQUIRITIGENIN ; PAEONIFLORIN
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:5380446
出版者	CHINESE JOURNAL NATURAL MEDICINES
WOS记录号	WOS:000355067700009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276615]
专题	上海中药现代化研究中心
通讯作者	Cui Ya-Jun
作者单位	1.Shanghai Univ Tradit Chinese Med, Sch Chinese Mat Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr TCM Modernizat, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wei Shan-Shan,Yang Min,Chen Xin,et al. Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2015,13(3):232-240.
APA	Wei Shan-Shan,Yang Min,Chen Xin,Wang Qiu-Rong,&Cui Ya-Jun.(2015).Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses.CHINESE JOURNAL OF NATURAL MEDICINES,13(3),232-240.
MLA	Wei Shan-Shan,et al."Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses".CHINESE JOURNAL OF NATURAL MEDICINES 13.3(2015):232-240.