Simultaneous quantitation of five Panax notoginseng saponins by multi heart-cutting two-dimensional liquid chromatography: Method development and application to the quality control of eight Notoginseng containing Chinese patent medicines

doi:10.1016/j.chroma.2015.05.015

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	Simultaneous quantitation of five Panax notoginseng saponins by multi heart-cutting two-dimensional liquid chromatography: Method development and application to the quality control of eight Notoginseng containing Chinese patent medicines
	Yao, Chang-liang 2; Yang, Wen-zhi 2; Wu, Wan-ying2 ; Da, Juan 2; Hou, Jin-jun2 ; Zhang, Jing-xian 2; Zhang, Yan-hai 1; Jin, Yan 1; Yang, Min 2; Jiang, Bao-hong2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2015-07-10
卷号	1402 页码:71-81
关键词	"Monomethod-heterotrait matrix" strategy Heart-cutting 2D-LC Dual-gradient liquid chromatography Chinese patent medicine Panax notoginseng Saponin analysis
ISSN号	0021-9673
DOI	10.1016/j.chroma.2015.05.015
文献子类	Article
英文摘要	Current China Pharmacopoeia (ChP) standards employ diversified and case-dependent assay methods to evaluate the quality of different Chinese patent medicines (CPMs) that contain Panax notoginseng as the monarch drug. These conventional, HPLC-based approaches, utilizing a complex sample preparation procedure, can easily result in low analytical efficiency and possible component loss. Here, a "monomethod-heterotrait matrix" (MHM) strategy is proposed, that is, developing a universal multi heart-cutting two-dimensional liquid chromatography (MHC-2D-LC) approach that facilitates the simultaneous quantitation of five P. notoginseng saponins (noto-R-1, Re, Rg(1), Rb-1, and Rd) in eight different CPMs. The MHC-2D-LC system was constructed on a dual-gradient liquid chromatography instrument equipped with a Poroshell SB C18 column and a Zorbax SB-Aq column for respective D-1 and D-2 separation. Method validation was performed in terms of specificity, linearity (r(2) and F-test), intra-/inter-day precision (0.4-7.9%), stability (1.2-3.9%), and recovery (90.2-108.7%), and the LODs and LOQs (loaded masses) of the five analytes varied between 4.0-11.0 ng and 6.0-33.0 ng, respectively. The validated MHC-2D-LC approach was subsequently applied to quantify the five saponins in thirty batches of different CPMs. The method demonstrated superiority over the current ChP assay methods in respect of specificity (avoiding co-elution), resolution (R-s > 1.5), sample preparation (easy-to-implement ultrasonic extraction without repeated re-extraction), and transfer rate (minimum component loss). This is the first application of an MHC-2D-LC method for the quantitative assessment of the constituents of CPMs. The MHM approach represents a new, strategically significant methodology for the quality control of CPMs that involve complex chemical matrix. (C) 2015 Elsevier B.V. All rights reserved.
资助项目	Standardization of Traditional Chinese Medicines/Indigenous Drugs - Chinese Academy of Sciences[KSZD-EW-Z-004-01] ; National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI291306] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002]
WOS关键词	TANDEM MASS-SPECTROMETRY ; NIUHUANG SHANGQING PILL ; DIFFERENT PARTS ; CHEMICAL CHARACTERISTICS ; HOLISTIC QUALITY ; PERFORMANCE ; CONSTITUENTS ; EXTRACTION ; MS ; SEPARATION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000356979900008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276470]
专题	上海中药现代化研究中心
通讯作者	Wu, Wan-ying
作者单位	1.Thermo Fisher Sci, Shanghai 201206, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Yao, Chang-liang,Yang, Wen-zhi,Wu, Wan-ying,et al. Simultaneous quantitation of five Panax notoginseng saponins by multi heart-cutting two-dimensional liquid chromatography: Method development and application to the quality control of eight Notoginseng containing Chinese patent medicines[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1402:71-81.
APA	Yao, Chang-liang.,Yang, Wen-zhi.,Wu, Wan-ying.,Da, Juan.,Hou, Jin-jun.,...&Guo, De-an.(2015).Simultaneous quantitation of five Panax notoginseng saponins by multi heart-cutting two-dimensional liquid chromatography: Method development and application to the quality control of eight Notoginseng containing Chinese patent medicines.JOURNAL OF CHROMATOGRAPHY A,1402,71-81.
MLA	Yao, Chang-liang,et al."Simultaneous quantitation of five Panax notoginseng saponins by multi heart-cutting two-dimensional liquid chromatography: Method development and application to the quality control of eight Notoginseng containing Chinese patent medicines".JOURNAL OF CHROMATOGRAPHY A 1402(2015):71-81.