Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual-wavelength fingerprinting mode and random forest

doi:10.1002/jssc.201500560

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual-wavelength fingerprinting mode and random forest
	Qiu, Yixing 1,2; Huang, Jianhua 1,3; Jiang, Xingming 1; Chen, Yang 1; Liu, Yang 1; Zeng, Rong 1; Shehla, Nuzhat 1; Liu, Qiang 1; Liao, Duanfang 2; Guo, Dean1,4
刊名	JOURNAL OF SEPARATION SCIENCE
	2015-11
卷号	38 期号:21 页码:3720-3726
关键词	Dual-wavelength fingerprinting Liquid chromatography LiuweiDihuang Quality control Random forest
ISSN号	1615-9306
DOI	10.1002/jssc.201500560
文献子类	Article
英文摘要	The classical traditional Chinese formulation LiuweiDihuang, shown to have clinical efficacy for "nourishing kidney-yin" in traditional Chinese medicine, has been used for thousands of years in China. Little attention, however, has been paid to quality control methods for this formulation. Hence, a rapid and sensitive analytical technique is urgently needed for the evaluation of LiuweiDihuang preparations to assess its quality and pharmacological functionality. In this study, an ultra high performance liquid chromatography dual-wavelength method was developed to simultaneously determine 11 constituents in LiuweiDihuang preparations. This robust approach provided a fast and comprehensive quantitative determination of the major bioactive markers within LiuweiDihuang preparations. To distinguish four dosage forms of LiuweiDihuang preparations, a random forest technique was applied on the spectrometric fingerprint data obtained. This combination approach of chromatographic techniques and data analyses might serve as a rapid and efficient tool to ensure the quality of LiuweiDihuang preparations and other Chinese medicinal formulations and can support quality control and scientific research into the pharmacological potential for these formulations.
资助项目	program for the Changsha Science & Technology Bureau[K1205019-31] ; Ministry of Education of Overseas Returnees Start-up Find for Scientific Research[2013693] ; Graduate Student Innovation Project of Hunan Province[CX2014B369]
WOS关键词	LIUWEI DIHUANG DECOCTION ; DIABETIC ENCEPHALOPATHY ; BIOACTIVE CONSTITUENTS ; STREPTOZOTOCIN ; RATS ; PILLS ; ACID ; DAD ; MS
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000363480800007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276348]
专题	上海中药现代化研究中心
通讯作者	Wang, Wei
作者单位	1.Hunan Univ Chinese Med, Sch Pharm, Sino Luxemburg TCM Res Ctr, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China; 2.Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat Chinese Mat Med, Changsha, Hunan, Peoples R China; 3.Cent S Univ, Res Ctr Modernizat Tradit Chinese Med, Changsha, Hunan, Peoples R China; 4.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Yixing,Huang, Jianhua,Jiang, Xingming,et al. Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual-wavelength fingerprinting mode and random forest[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(21):3720-3726.
APA	Qiu, Yixing.,Huang, Jianhua.,Jiang, Xingming.,Chen, Yang.,Liu, Yang.,...&Wang, Wei.(2015).Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual-wavelength fingerprinting mode and random forest.JOURNAL OF SEPARATION SCIENCE,38(21),3720-3726.
MLA	Qiu, Yixing,et al."Quantitative and qualitative determination of LiuweiDihuang preparations by ultra high performance liquid chromatography in dual-wavelength fingerprinting mode and random forest".JOURNAL OF SEPARATION SCIENCE 38.21(2015):3720-3726.