Nano-LC-ESI MS/MS analysis of proteins in dried sea dragon Solenognathus hardwickii and bioinformatic analysis of its protein expression profiling

doi:10.1016/S1875-5364(16)30084-X

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	Nano-LC-ESI MS/MS analysis of proteins in dried sea dragon Solenognathus hardwickii and bioinformatic analysis of its protein expression profiling
	Zhang Dong-Mei 1,2; Feng Li-Xing 2; Li Lu 2; Liu Miao 2; Jiang Bao-Hong2 ; Yang Min 2; Li Guo-Qiang 3; Wu Wan-Ying2 ; Guo De-An2 ; Liu Xuan 2
刊名	CHINESE JOURNAL OF NATURAL MEDICINES
	2016-09
卷号	14 期号:9 页码:709-713
关键词	Sea dragon Solenognathus hardwickii Mass-spectrometry-based proteomics Bioinformatic analysis
ISSN号	2095-6975
DOI	10.1016/S1875-5364(16)30084-X
文献子类	Article
英文摘要	The sea dragon Solenognathus hardwickii has long been used as a traditional Chinese medicine for the treatment of various diseases, such as male impotency. To gain a comprehensive insight into the protein components of the sea dragon, shotgun proteomic analysis of its protein expression profiling was conducted in the present study. Proteins were extracted from dried sea dragon using a trichloroacetic acid/acetone precipitation method and then separated by SDS-PAGE. The protein bands were cut from the gel and digested by trypsin to generate peptide mixture. The peptide fragments were then analyzed using nano liquid chromatography tandem mass spectrometry (nano-LC-ESI MS/MS). 810 proteins and 1 577 peptides were identified in the dried sea dragon. The identified proteins exhibited molecular weight values ranging from 1 900 to 3 516 900 Da and pI values from 3.8 to 12.18. Bioinformatic analysis was conducted using the DAVID Bioinformatics Resources 6.7 Gene Ontology (GO) analysis tool to explore possible functions of the identified proteins. Ascribed functions of the proteins mainly included intracellular non-membrane-bound organelle, non-membrane-bounded organelle, cytoskeleton, structural molecule activity, calcium ion binding and etc. Furthermore, possible signal networks of the identified proteins were predicted using STRING (Search Tool for the Retrieval of Interacting Genes) database. Ribosomal protein synthesis was found to play an important role in the signal network. The results of this study, to best of our knowledge, were the first to provide a reference proteome profile for the sea dragon, and would aid in the understanding of the expression and functions of the identified proteins.
资助项目	National High Technology Research and Development Program of China[2013AA093002] ; Shanghai Science & Technology Support Program[13431900401] ; Shanghai Science & Technology Innovation Action Program[15140904800] ; National Nature Science Foundation of China[81373964] ; National Science & Technology Major Project of China[2014ZX09301-306-03]
WOS关键词	PROTEOMIC ANALYSIS ; FISH
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:5806197
出版者	CHINESE JOURNAL NATURAL MEDICINES
WOS记录号	WOS:000386599300009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275905]
专题	上海中药现代化研究中心
通讯作者	Wu Wan-Ying; Liu Xuan
作者单位	1.Lanzhou Mil Command Chinese PLA, Lanzhou Gen Hosp, Dept Pharm, Lanzhou 730050, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat TCM, Shanghai 201203, Peoples R China; 3.Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Chinese Minist Educ, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Zhang Dong-Mei,Feng Li-Xing,Li Lu,et al. Nano-LC-ESI MS/MS analysis of proteins in dried sea dragon Solenognathus hardwickii and bioinformatic analysis of its protein expression profiling[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2016,14(9):709-713.
APA	Zhang Dong-Mei.,Feng Li-Xing.,Li Lu.,Liu Miao.,Jiang Bao-Hong.,...&Liu Xuan.(2016).Nano-LC-ESI MS/MS analysis of proteins in dried sea dragon Solenognathus hardwickii and bioinformatic analysis of its protein expression profiling.CHINESE JOURNAL OF NATURAL MEDICINES,14(9),709-713.
MLA	Zhang Dong-Mei,et al."Nano-LC-ESI MS/MS analysis of proteins in dried sea dragon Solenognathus hardwickii and bioinformatic analysis of its protein expression profiling".CHINESE JOURNAL OF NATURAL MEDICINES 14.9(2016):709-713.