Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum | |
Ma, Chao; Guan, Shu-Hong![]() ![]() | |
刊名 | PHYTOMEDICINE
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2008-04 | |
卷号 | 15期号:4页码:268-276 |
关键词 | Ganoderma lucidum polysaccharides proteomics mononuclear cell immuno-modulation |
ISSN号 | 0944-7113 |
DOI | 10.1016/j.phymed.2007.11.015 |
文献子类 | Article |
英文摘要 | Polysaccharides were one of the main components of Ganoderma lucidum, a medicinal mushroom well known for its immuno-modulation effects. In the present study, we demonstrated that polysaccharides extracted from Ganoderma lucidum spores (GL-SP) could stimulate splenic mononuclear cells (MNCs) proliferation and cytokine production. To identify the possible cellular targets of GL-SP in MNCs, two-dimensional gel electrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after GL-SP treatment were identified by MALDI-TOF MS/MS. Ten proteins with > 2-fold increase or decrease expression in GL-SP-treated MNCs compared with control were found and further identified by MALDI-TOF MS/MS analysis and database searching. In the GL-SP-treated MNCs, there were increases in the expression of myosin regulatory light chain 2-A, Rho GDP dissociation inhibitor beta, T-cell-specific GTPase, phosphatidylinositol transfer protein a, and decreases in the expression of apoptosis-associated speck-like protein containing a CARD, 14-3-3 tau, beta-actin, tubulin alpha 2, copine 1, and gamma-actin. The results of the present study help to provide insight into the immuno-modulating activities of GL-SP. (C) 2007 Elsevier GmbH. All rights reserved. |
WOS关键词 | MACROPHAGES ; ACTIVATION ; ALPHA |
WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
语种 | 英语 |
出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
WOS记录号 | WOS:000255889800006 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/272952] ![]() |
专题 | 上海中药现代化研究中心 |
通讯作者 | Liu, Xuan |
作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Chao,Guan, Shu-Hong,Yang, Min,et al. Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum[J]. PHYTOMEDICINE,2008,15(4):268-276. |
APA | Ma, Chao,Guan, Shu-Hong,Yang, Min,Liu, Xuan,&Guo, De-An.(2008).Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum.PHYTOMEDICINE,15(4),268-276. |
MLA | Ma, Chao,et al."Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum".PHYTOMEDICINE 15.4(2008):268-276. |
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