Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum

doi:10.1016/j.phymed.2007.11.015

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	Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum
	Ma, Chao; Guan, Shu-Hong; Yang, Min; Liu, Xuan; Guo, De-An
刊名	PHYTOMEDICINE
	2008-04
卷号	15 期号:4 页码:268-276
关键词	Ganoderma lucidum polysaccharides proteomics mononuclear cell immuno-modulation
ISSN号	0944-7113
DOI	10.1016/j.phymed.2007.11.015
文献子类	Article
英文摘要	Polysaccharides were one of the main components of Ganoderma lucidum, a medicinal mushroom well known for its immuno-modulation effects. In the present study, we demonstrated that polysaccharides extracted from Ganoderma lucidum spores (GL-SP) could stimulate splenic mononuclear cells (MNCs) proliferation and cytokine production. To identify the possible cellular targets of GL-SP in MNCs, two-dimensional gel electrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after GL-SP treatment were identified by MALDI-TOF MS/MS. Ten proteins with > 2-fold increase or decrease expression in GL-SP-treated MNCs compared with control were found and further identified by MALDI-TOF MS/MS analysis and database searching. In the GL-SP-treated MNCs, there were increases in the expression of myosin regulatory light chain 2-A, Rho GDP dissociation inhibitor beta, T-cell-specific GTPase, phosphatidylinositol transfer protein a, and decreases in the expression of apoptosis-associated speck-like protein containing a CARD, 14-3-3 tau, beta-actin, tubulin alpha 2, copine 1, and gamma-actin. The results of the present study help to provide insight into the immuno-modulating activities of GL-SP. (C) 2007 Elsevier GmbH. All rights reserved.
WOS关键词	MACROPHAGES ; ACTIVATION ; ALPHA
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
WOS记录号	WOS:000255889800006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272952]
专题	上海中药现代化研究中心
通讯作者	Liu, Xuan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Ma, Chao,Guan, Shu-Hong,Yang, Min,et al. Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum[J]. PHYTOMEDICINE,2008,15(4):268-276.
APA	Ma, Chao,Guan, Shu-Hong,Yang, Min,Liu, Xuan,&Guo, De-An.(2008).Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum.PHYTOMEDICINE,15(4),268-276.
MLA	Ma, Chao,et al."Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum".PHYTOMEDICINE 15.4(2008):268-276.