Quantitative determination of ganoderic acid H in SunRecome by Reversed-phase high performance chromatography

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	Quantitative determination of ganoderic acid H in SunRecome by Reversed-phase high performance chromatography
	WANG Xiaoming 2; GUAN Shuhong1 ; LIU Rongxia 1; CHEN Guangtong 2; BI Kaishun 2; GUO Dean1
刊名	Journal of Shenyang Pharmaceutical University
	2007
卷号	24 期号:7 页码:407
关键词	SunRecome (traditional Chinese medicines) ganoderic arid H RP-HPLC quantitative determination
ISSN号	1006-2858
其他题名	RP-HPLC法测定双灵固本散中灵芝酸H的含量
文献子类	Article
英文摘要	Objective To develop a RP-HPLC method for the quantitative determination of ganoderic acid H in the SunRecome(traditional Chinese medicines). Methods The chromatographic separation was achieved on an Agilent Zorbax SB-C_(18) column (250 mm * 4.6 mm, 5 mum) at 35℃, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid(psi), at a flow rate of 1.0 mL·min~(-1). The detecting wavelength was set at 252 nm.Results The calibration curves showed good linearity(r < 0.999) within 16.34-408.5 kg·L~(-1). The average recovery of ganoderic acid H was 99.0% with RSD of 2.6% (n = 9). This method was successfully applied for analyzing 10 batches of SunRecome. The contents of ganoderic acid H were ranged from 0.130% to 0.348%. Conclusions The method is sensitive, accurate, reliable and suitable for the quality control of the SunRecome.
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:2838341
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268147]
专题	上海中药现代化研究中心
通讯作者	GUO Dean
作者单位	1.Shanghai Research Center for TCM Modernization, Shanghai Institute of Materia Medica, Shanghai Institutse for Biological Sciences, Shanghai 201203, China. 2.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.;
推荐引用方式 GB/T 7714	WANG Xiaoming,GUAN Shuhong,LIU Rongxia,et al. Quantitative determination of ganoderic acid H in SunRecome by Reversed-phase high performance chromatography[J]. Journal of Shenyang Pharmaceutical University,2007,24(7):407.
APA	WANG Xiaoming,GUAN Shuhong,LIU Rongxia,CHEN Guangtong,BI Kaishun,&GUO Dean.(2007).Quantitative determination of ganoderic acid H in SunRecome by Reversed-phase high performance chromatography.Journal of Shenyang Pharmaceutical University,24(7),407.
MLA	WANG Xiaoming,et al."Quantitative determination of ganoderic acid H in SunRecome by Reversed-phase high performance chromatography".Journal of Shenyang Pharmaceutical University 24.7(2007):407.