Studies on HPLC -ELSD specific fingerprints of Rhizoma  Anemarrhenae of different sources

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	Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources
	KE Wei 1; MA Chunhui 2; ZHANG Rujuan 2; SUN Zhaolin 2; JI Yubin 1; HUANG Chenggang2
刊名	Chinese Journal of Pharmaceutical Analysis
	2008
卷号	28 期号:1 页码:100-103
关键词	HPLCELSD Rhizoma Anemarrhenae HPLC - ELSD fingerprint
ISSN号	0254-1793
其他题名	不同来源知母药材HPLC-ELSD指纹图谱的研究
文献子类	Article
英文摘要	Objective： To establish the HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different sources. Method： The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different sources were obtained by Waters 600 instruments. The separation was performed on C_(18) analytical column（200 mm * 4. 6 mm ,5 mum）gradient eluted with a mixture consisting of acetonitrile（A） and 0. 5% formic acid solution（B） at a flow rate of 0. 8 mL · min ~(-1) ,using a linear gradient of 5% A20% A during 0 - 10 min;20% A23% A during 10 - 15 min;23% A during 15 -25 min;23% A100% A during 25 -50 min;100% A during 50 -60 min. The temperature of column was 25 ℃, The temperature of drift tube was 110 ℃, and the nebulizer nitrogen flow rate was 2. 6 L· min~(-1). Result：The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae were established, and the similarity of medicinal materials from different sources was compared. Furthermore, four peaks in HPLC - ELSD chromatogram were firsdy assigned as timosaponin B -Ⅱ , timosaponin B - Ⅲ, anemarrhenasaponin Ⅰ a, schidigera - saponin - F2. Conclusion： The method is simple,reproducible and can be used as a quality control of Rhizoma Anemarrhenae.
资助项目	中国科学院重要方向项目的部分研究内容（项目编号：KGCX2-SW-213-03）[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:3224481
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268101]
专题	上海中药现代化研究中心
通讯作者	HUANG Chenggang
作者单位	1.Center of Research and Development on Life Sciences and Environmental Sciences, Herhin University of Commerce, Herbin, 150076.; 2.Shanghai Institute of Materia Medica, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	KE Wei,MA Chunhui,ZHANG Rujuan,et al. Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources[J]. Chinese Journal of Pharmaceutical Analysis,2008,28(1):100-103.
APA	KE Wei,MA Chunhui,ZHANG Rujuan,SUN Zhaolin,JI Yubin,&HUANG Chenggang.(2008).Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources.Chinese Journal of Pharmaceutical Analysis,28(1),100-103.
MLA	KE Wei,et al."Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources".Chinese Journal of Pharmaceutical Analysis 28.1(2008):100-103.