Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources | |
KE Wei1; MA Chunhui2; ZHANG Rujuan2; SUN Zhaolin2; JI Yubin1; HUANG Chenggang2![]() | |
刊名 | Chinese Journal of Pharmaceutical Analysis
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2008 | |
卷号 | 28期号:1页码:100-103 |
关键词 | HPLCELSD Rhizoma Anemarrhenae HPLC - ELSD fingerprint |
ISSN号 | 0254-1793 |
其他题名 | 不同来源知母药材HPLC-ELSD指纹图谱的研究 |
文献子类 | Article |
英文摘要 | Objective: To establish the HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different sources. Method: The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different sources were obtained by Waters 600 instruments. The separation was performed on C_(18) analytical column(200 mm * 4. 6 mm ,5 mum)gradient eluted with a mixture consisting of acetonitrile(A) and 0. 5% formic acid solution(B) at a flow rate of 0. 8 mL · min ~(-1) ,using a linear gradient of 5% A20% A during 0 - 10 min;20% A23% A during 10 - 15 min;23% A during 15 -25 min;23% A100% A during 25 -50 min;100% A during 50 -60 min. The temperature of column was 25 ℃, The temperature of drift tube was 110 ℃, and the nebulizer nitrogen flow rate was 2. 6 L· min~(-1). Result:The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae were established, and the similarity of medicinal materials from different sources was compared. Furthermore, four peaks in HPLC - ELSD chromatogram were firsdy assigned as timosaponin B -Ⅱ , timosaponin B - Ⅲ, anemarrhenasaponin Ⅰ a, schidigera - saponin - F2. Conclusion: The method is simple,reproducible and can be used as a quality control of Rhizoma Anemarrhenae. |
资助项目 | 中国科学院重要方向项目的部分研究内容(项目编号:KGCX2-SW-213-03)[00000000] |
WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
语种 | 中文 |
CSCD记录号 | CSCD:3224481 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/268101] ![]() |
专题 | 上海中药现代化研究中心 |
通讯作者 | HUANG Chenggang |
作者单位 | 1.Center of Research and Development on Life Sciences and Environmental Sciences, Herhin University of Commerce, Herbin, 150076.; 2.Shanghai Institute of Materia Medica, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai 201203, China. |
推荐引用方式 GB/T 7714 | KE Wei,MA Chunhui,ZHANG Rujuan,et al. Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources[J]. Chinese Journal of Pharmaceutical Analysis,2008,28(1):100-103. |
APA | KE Wei,MA Chunhui,ZHANG Rujuan,SUN Zhaolin,JI Yubin,&HUANG Chenggang.(2008).Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources.Chinese Journal of Pharmaceutical Analysis,28(1),100-103. |
MLA | KE Wei,et al."Studies on HPLC -ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources".Chinese Journal of Pharmaceutical Analysis 28.1(2008):100-103. |
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