Study on proteomics of HeLa cell apoptosis in bufalin-induced human cervical carcinoma

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	Study on proteomics of HeLa cell apoptosis in bufalin-induced human cervical carcinoma
	Pan Suna 3; Wang Yuhong 1; Feng Lixing 2; Fan Cuiying 3; Guo Dean2 ; Liu Xuan 2; Fan Jinling 3
刊名	China Journal of Chinese Materia Medica
	2012
卷号	37 期号:13 页码:1998-2004
关键词	bufalin HeLa cell cell apoptosis two-dimensional electrophoresis proteomics
ISSN号	1001-5302
其他题名	蟾毒灵诱导人宫颈癌HeLa细胞凋亡的蛋白组学研究
文献子类	Article
英文摘要	Objective: To seek possible effect targets of bufalin in HeLa cells by studying the impact of bufalin on cell protein expression profile after treatment on human cervical carcinoma cell lines HeLa.Method: Bufalin′s IC_(50) was measured by MTT assay.The apoptosis of cells was observed by FCM(flow cytometry) and Hoechst 33342 staining assay.Differentiated expression protein spots were founded and identified using proteomic techniques,which could induce HeLa cell apoptosis.Result: Bufalin showed remarkable cytotoxic effect on HeLa cells.IC_(50)(15421.5) nmol·L~(-1) indicated the possibility of inducing cell apoptosis.The protein expression profile showed 11 differentiated expression protein spots.Among the 11 proteins,nudix-type motif 5,vimentin,hnRNP C1/hnRNP C2 variant,HNRPK,HNRPK isoform a variant(two spots are the same protein),heat shock protein 27,macrophage-capping protein,SELENBP1 protein were down-regulated,while ribosomal protein,large,P0 and S-adenosylmethionine synthetase 2 were up-regulated by bufalin treatment.They may be effect targets of bufalin in HeLa cells.Western blotting showed consistent results in heat shock protein 27,vimentin and HNRPK between expression after treatment with bufalin and two-dimensional electrophoresis.Conclusion: Bufalin can induce apoptosis in human cervical carcinoma cells HeLa and the effect of bufalin may be related to the joint intervention with multiple protein targets.
资助项目	中国科学院知识创新工程重大项目[00000000] ; 十二五国家科技支撑计划[00000000]
WOS研究方向	General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:4607733
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267825]
专题	上海中药现代化研究中心
通讯作者	Guo Dean
作者单位	1.Liaoning Radio and Television University, Shenyang, Liaoning 110034, China.; 2.The Chinese Academy of Sciences,Shanghai Institutes of Materia Medica, Shanghai 201203, China. 3.College of Food and Bioengineering,Henan University of Science and Technology, Luoyang, Henan 471003, China.;
推荐引用方式 GB/T 7714	Pan Suna,Wang Yuhong,Feng Lixing,et al. Study on proteomics of HeLa cell apoptosis in bufalin-induced human cervical carcinoma[J]. China Journal of Chinese Materia Medica,2012,37(13):1998-2004.
APA	Pan Suna.,Wang Yuhong.,Feng Lixing.,Fan Cuiying.,Guo Dean.,...&Fan Jinling.(2012).Study on proteomics of HeLa cell apoptosis in bufalin-induced human cervical carcinoma.China Journal of Chinese Materia Medica,37(13),1998-2004.
MLA	Pan Suna,et al."Study on proteomics of HeLa cell apoptosis in bufalin-induced human cervical carcinoma".China Journal of Chinese Materia Medica 37.13(2012):1998-2004.