A Smart Nanoassembly Consisting of Acid-Labile Vinyl Ether PEG-DOPE and Protamine for Gene Delivery: Preparation and in Vitro Transfection

doi:10.1021/bm800706f

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物制剂研究中心

	A Smart Nanoassembly Consisting of Acid-Labile Vinyl Ether PEG-DOPE and Protamine for Gene Delivery: Preparation and in Vitro Transfection
	Xu, Zhenghong; Gu, Wangwen; Chen, Lingli; Gao, Yu; Zhang, Zhiwen; Li, Yaping
刊名	BIOMACROMOLECULES
	2008-11
卷号	9 期号:11 页码:3119-3126
ISSN号	1525-7797
DOI	10.1021/bm800706f
文献子类	Article
英文摘要	The conception of a modular designed and viruslike nonviral vector has been presented for gene delivery. Recently, we constructed a new smart nanoassembly (SNA) with multifunctional components that was composed of a condensed core of pDNA with prolamine sulfate (PS) and a dioleoyl phosphatidylethanolamine (DOPE)-based lipid envelope containing poly(ethylene glycol)-vinyl ether-DOPE (PVD). SNAs with mPEG 2000 (SNAs I) or mPEG 5000 (SNAs2) loading PS/DNA were prepared by the lipid film hydration technique. The particle size was about 160 nm for SNAs1 and 240 nm for SNAs2 loading PS/DNA (10:1 w/w), and the zeta potential was about 4 mV for two SNAs. The in vitro release experiment indicated that PVD possessed a good ability for self-dePEGylation, which could result in the recovery of an excellent fusogenic capacity of DOPE at low pH. SNAs showed a higher transfection efficiency and much lower cytotoxicity than did Lipofectamine 2000 on HEK 293, HeLa. and COS-7 cells. The cellular uptake and subcellular localization demonstrated that the superior transfection efficiency of SNAs could result from the fact that the DOPE-based lipid envelope containing PVD increased PS/DNA in the cytoplasm, and protamine enhanced the nuclear delivery or overcame the nuclear membrane barrier. These results implied that the PVD-based nanoassembly loading PS/DNA could be a promising gene delivery system.
资助项目	National Basic Research Program of China[2007CB935804] ; National Basic Research Program of China[2004CB518802] ; National Natural Science Foundation of China[30572259] ; Important Direction Program of CAS[KSCX2-YWR-09]
WOS关键词	PH-SENSITIVE LIPOSOMES ; DNA LPD COMPLEXES ; DRUG-DELIVERY ; TRIGGERED RELEASE ; PLASMID DNA ; FUSOGENIC LIPOSOMES ; NANO DEVICE ; NANOPARTICLES ; SYSTEM ; VIVO
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000260856900021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279443]
专题	药物制剂研究中心
通讯作者	Li, Yaping
作者单位	Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhenghong,Gu, Wangwen,Chen, Lingli,et al. A Smart Nanoassembly Consisting of Acid-Labile Vinyl Ether PEG-DOPE and Protamine for Gene Delivery: Preparation and in Vitro Transfection[J]. BIOMACROMOLECULES,2008,9(11):3119-3126.
APA	Xu, Zhenghong,Gu, Wangwen,Chen, Lingli,Gao, Yu,Zhang, Zhiwen,&Li, Yaping.(2008).A Smart Nanoassembly Consisting of Acid-Labile Vinyl Ether PEG-DOPE and Protamine for Gene Delivery: Preparation and in Vitro Transfection.BIOMACROMOLECULES,9(11),3119-3126.
MLA	Xu, Zhenghong,et al."A Smart Nanoassembly Consisting of Acid-Labile Vinyl Ether PEG-DOPE and Protamine for Gene Delivery: Preparation and in Vitro Transfection".BIOMACROMOLECULES 9.11(2008):3119-3126.