Galactosylated Poly(2-(2-aminoethyoxy)ethoxy)phosphazene/DNA Complex Nanoparticles: In Vitro and In Vivo Evaluation for Gene Delivery

doi:10.1021/bm901346m

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	Galactosylated Poly(2-(2-aminoethyoxy)ethoxy)phosphazene/DNA Complex Nanoparticles: In Vitro and In Vivo Evaluation for Gene Delivery
	Yang, Yongxin; Zhang, Zhiwen; Chen, Lingli; Gu, Wangwen; Li, Yaping
刊名	BIOMACROMOLECULES
	2010-04
卷号	11 期号:4 页码:927-933
ISSN号	1525-7797
DOI	10.1021/bm901346m
文献子类	Article
英文摘要	To achieve efficient gene delivery to the tumor after intravenous administration, biodegradable poly(2-(2aminoethyoxy)ethoxy)phosphazene (PAEP) was modified by lactobionic acid, bearing a galactose group as a targeting ligand. Galactosylated poly(2-(2-aminoethyoxy)ethoxy)phosphazene (Gal-PAEP) with 4.9% substitution degree of galactose could condense pDNA into nanoparticles with a size around 130 nm at the polymer/DNA ratio (N/P) of 2-40. For BEL-7402 cells, the in vitro transfection efficiency of gal-PAEP/DNA complex nanoparticles (gal-PACNs) was much higher than that of the PAEP/DNA complex nanoparticles (PACNs). MTT assay indicated that the cytotoxicity of PACNs significantly decreased after conjugating with the galactose moiety. Gal-PACNs displayed the selective gene expression in the tumor and liver with relatively low gene expression in the lung or other organs compared with PACNs. These results suggested that gal-PACNs could be a promising targeting gene carrier to deliver a therapeutic gene in future.
资助项目	National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[30901866] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-024] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; Shanghai Nanomedicine Program[0852 nm05700]
WOS关键词	TRANSFECTION EFFICIENCY ; CATIONIC LIPOSOMES ; HEPG2 CELLS ; DNA ; CARRIER ; HEPATOCYTES ; CHITOSAN ; POLYETHYLENIMINE ; ENDOCYTOSIS ; POLYPLEXES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000276557300013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278925]
专题	药物制剂研究中心
通讯作者	Li, Yaping
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yongxin,Zhang, Zhiwen,Chen, Lingli,et al. Galactosylated Poly(2-(2-aminoethyoxy)ethoxy)phosphazene/DNA Complex Nanoparticles: In Vitro and In Vivo Evaluation for Gene Delivery[J]. BIOMACROMOLECULES,2010,11(4):927-933.
APA	Yang, Yongxin,Zhang, Zhiwen,Chen, Lingli,Gu, Wangwen,&Li, Yaping.(2010).Galactosylated Poly(2-(2-aminoethyoxy)ethoxy)phosphazene/DNA Complex Nanoparticles: In Vitro and In Vivo Evaluation for Gene Delivery.BIOMACROMOLECULES,11(4),927-933.
MLA	Yang, Yongxin,et al."Galactosylated Poly(2-(2-aminoethyoxy)ethoxy)phosphazene/DNA Complex Nanoparticles: In Vitro and In Vivo Evaluation for Gene Delivery".BIOMACROMOLECULES 11.4(2010):927-933.