Mesoporous silica nanoparticles loading doxorubicin reverse multidrug resistance: performance and mechanism | |
Shen, Jianan2; He, Qianjun1; Gao, Yu2; Shi, Jianlin1; Li, Yaping2![]() | |
刊名 | NANOSCALE
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2011 | |
卷号 | 3期号:10页码:4314-4322 |
ISSN号 | 2040-3364 |
DOI | 10.1039/c1nr10580a |
文献子类 | Article |
英文摘要 | Multidrug resistance (MDR) is one of the major obstacles for successful chemotherapy in cancer. One of the effective approaches to overcome MDR is to use nanoparticle-mediated drug delivery to increase drug accumulation in drug resistant cancer cells. In this work, we first report that the performance and mechanism of an inorganic engineered delivery system based on mesoporous silica nanoparticles (MSNs) loading doxorubicin (DMNs) to overcome the MDR of MCF-7/ADR (a DOX-resistant and P-glycoprotein (P-gp) over-expression cancer cell line). The experimental results showed that DMNs could enhance the cellular uptake of doxorubicin (DOX) and increase the cell proliferation suppression effect of DOX against MCF-7/ADR cells. The IC(50) of DMNs against MCF-7/ADR cells was 8-fold lower than that of free DOX. However, an improved effect of DOX in DMNs against MCF-7 cells (a DOX-sensitive cancer cell line) was not found. The increased cellular uptake and nuclear accumulation of DOX delivered by DMNs in MCF-7/ADR cells was confirmed by confocal laser scanning microscopy, and could result from the down-regulation of P-gp and bypassing the efflux action by MSNs themselves. The cellular uptake mechanism of DMNs indicated that the macropinocytosis was one of the pathways for the uptake of DMNs by MCF-7/ADR cells. The in vivo biodistribution showed that DMNs induced a higher accumulation of DOX in drug resistant tumors than free DOX. These results suggested that MSNs could be an effective delivery system to overcome multidrug resistance. |
资助项目 | The National Basic Research Program of China[2010CB934000] ; The National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[50823007] ; Science and Technology Commission of Shanghai[10430712800] ; Science Foundation for Youth Scholar of State Key Laboratory of High Performance Ceramics and Superfine Microstructures[SKL201001] |
WOS关键词 | DRUG-DELIVERY SYSTEMS ; IN-VIVO ; MAGNETIC NANOPARTICLES ; CONTROLLED-RELEASE ; CANCER-CELLS ; CARRIERS ; SPHERES ; CYTOTOXICITY ; LIPOSOMES ; POLYMERS |
WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000295618200049 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/278666] ![]() |
专题 | 药物制剂研究中心 |
通讯作者 | Shi, Jianlin |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Shen, Jianan,He, Qianjun,Gao, Yu,et al. Mesoporous silica nanoparticles loading doxorubicin reverse multidrug resistance: performance and mechanism[J]. NANOSCALE,2011,3(10):4314-4322. |
APA | Shen, Jianan,He, Qianjun,Gao, Yu,Shi, Jianlin,&Li, Yaping.(2011).Mesoporous silica nanoparticles loading doxorubicin reverse multidrug resistance: performance and mechanism.NANOSCALE,3(10),4314-4322. |
MLA | Shen, Jianan,et al."Mesoporous silica nanoparticles loading doxorubicin reverse multidrug resistance: performance and mechanism".NANOSCALE 3.10(2011):4314-4322. |
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