Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism

doi:10.2147/IJN.S24720

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物制剂研究中心

	Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
	Zhang, Zhiwen1,2,3 ; Fang, Xiaoling 2,3; Hao, Junguo 2,3; Li, Yajuan 2,3; Sha, Xianyi 2,3
刊名	INTERNATIONAL JOURNAL OF NANOMEDICINE
	2011
卷号	6 页码:2235-2244
关键词	triolein dioleoyl phosphatidylethanolalmine polyethylenimine lipid nanocarrier mechanism
ISSN号	1178-2013
DOI	10.2147/IJN.S24720
文献子类	Article
英文摘要	We proposed to develop a polycation lipid nanocarrier (PLN) with higher - transfection efficiency than our previously described polycation nanostrucutred lipid nanocarrier (PNLC). PLN was composed of triolein, cetylated low-molecular-weight polyethylenimine, and dioleoyl phosphatidylethanolamine. The physicochemical properties of PLN and the PLN/DNA complexes (PDC) were characterized. The in vitro transfection was performed in human lung adenocarcinoma (SPC-A1) cells, and the intracellular mechanism was investigated as well. The measurements indicated that PLN and PDC are homogenous nanometer-sized particles with a positive charge. The transfection efficiency of PDC significantly increased with the content of triolein and was higher than that of PNLC and commercial Lipofectamine (TM) 2000. In particular, the transfection of PLN in the presence of 10% serum was more effective than that in its absence. With the help of specific inhibitors of chlorpromazine and filipin, the clathrin-dependent endocytosis pathway was determined to be the main contributor to the successful transfection mediated by PLN in SPC-A1 cells. The captured images verified that the fluorescent PDC was localized in the lysosomes and nuclei after endocytosis. Thus, PLN represents a novel efficient nonviral gene delivery vector.
资助项目	National Natural Science Foundation of China[30901862] ; National Natural Science Foundation of China[30901866] ; National Natural Science Foundation of China[30873177] ; Shanghai Rising-Star Program[10QA1400800] ; Shanghai Rising-Star Program[11QA1407900] ; Scientific Research Foundation for the Returned Overseas Chinese Scholar[00000000]
WOS关键词	NONVIRAL VECTOR ; TRANSFER SYSTEM ; DNA VACCINE ; POLYETHYLENIMINE ; LIPOSOMES ; TRANSFECTION ; COMPLEXES ; ENDOCYTOSIS ; EXPRESSION ; TOXICITY
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
语种	英语
出版者	DOVE MEDICAL PRESS LTD
WOS记录号	WOS:000297676300001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278648]
专题	药物制剂研究中心
通讯作者	Sha, Xianyi
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Fudan Univ, Dept Pharmaceut, Sch Pharm, Key Lab Smart Drug Delivery,Minist Educ, Shanghai 201203, Peoples R China; 3.Fudan Univ, PLA, Sch Pharm, Dept Pharmaceut, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Zhiwen,Fang, Xiaoling,Hao, Junguo,et al. Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2011,6:2235-2244.
APA	Zhang, Zhiwen,Fang, Xiaoling,Hao, Junguo,Li, Yajuan,&Sha, Xianyi.(2011).Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism.INTERNATIONAL JOURNAL OF NANOMEDICINE,6,2235-2244.
MLA	Zhang, Zhiwen,et al."Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism".INTERNATIONAL JOURNAL OF NANOMEDICINE 6(2011):2235-2244.