Simultaneous inhibition of metastasis and growth of breast cancer by co-delivery of twist shRNA and paclitaxel using pluronic P85-PEI/TPGS complex nanoparticles

doi:10.1016/j.biomaterials.2012.10.057

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	Simultaneous inhibition of metastasis and growth of breast cancer by co-delivery of twist shRNA and paclitaxel using pluronic P85-PEI/TPGS complex nanoparticles
	Shen, Jianan 2; Sun, Huiping 1,2; Xu, Pengfei 2; Yin, Qi2 ; Zhang, Zhiwen2 ; Wang, Siling 1; Yu, Haijun2 ; Li, Yaping2
刊名	BIOMATERIALS
	2013-02
卷号	34 期号:5 页码:1581-1590
关键词	Metastasis RNA Paclitaxel Twist Pluronic P85
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2012.10.057
文献子类	Article
英文摘要	The metastasis of breast cancer is the leading cause of cancer death in women, and the lung is a common location of a secondary tumor that has metastasized from the primary source tumor. In this work, an attempt to simultaneously inhibit the metastasis and growth of tumor by co-delivering Twist shRNA (shTwi) and paclitaxel (PTX) using the conjugate of pluronic P85 (P85) and low molecular weight polyethyleneimine (PEI) (P85-PEI)/D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) complex nanoparticles (PTPNs) was performed on metastatic 4T1 breast cancer cell line and its pulmonary metastasis mice model. The experimental results demonstrated that PTPNs could effectively achieve cellular uptake and RNA interference. The down-regulation of Twist protein resulted in significant inhibitory effect of cell migration and invasion with the inhibition rate of 88.7% and 91.06%, respectively. The IC50 of PTPNs against 4T1 cells was 63-fold lower than that of free PTX. The prolonged circulation and increased accumulation of PTX and shTwi in lung and tumor were observed in in vivo biodistribution. The in vivo antitumor efficacy showed that PTPNs could not only inhibit the in situ tumor growth effectively, but also completely restrict the pulmonary metastasis in 4T1 pulmonary metastatic mice model. Therefore, co-delivering chemotherapy drugs with metastasis regulator by PTPNs to simultaneously inhibit metastasis and growth of tumor could achieve synergistic effect for the effective therapy of metastatic breast cancer. (C) 2012 Elsevier Ltd. All rights reserved.
资助项目	National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2012CB932502] ; National Basic Research Program of China[2009CB930304] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[81270047] ; Shanghai Elitist Program[11XD1406200]
WOS关键词	MULTIDRUG-RESISTANT CANCER ; OVERCOME DRUG-RESISTANCE ; POLYMERIC MICELLES ; BLOCK-COPOLYMERS ; ANTICANCER DRUG ; SURVIVIN SHRNA ; GENE DELIVERY ; IN-VIVO ; TUMOR ; NANOCARRIERS
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000313929400014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277753]
专题	药物制剂研究中心
通讯作者	Li, Yaping
作者单位	1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Shen, Jianan,Sun, Huiping,Xu, Pengfei,et al. Simultaneous inhibition of metastasis and growth of breast cancer by co-delivery of twist shRNA and paclitaxel using pluronic P85-PEI/TPGS complex nanoparticles[J]. BIOMATERIALS,2013,34(5):1581-1590.
APA	Shen, Jianan.,Sun, Huiping.,Xu, Pengfei.,Yin, Qi.,Zhang, Zhiwen.,...&Li, Yaping.(2013).Simultaneous inhibition of metastasis and growth of breast cancer by co-delivery of twist shRNA and paclitaxel using pluronic P85-PEI/TPGS complex nanoparticles.BIOMATERIALS,34(5),1581-1590.
MLA	Shen, Jianan,et al."Simultaneous inhibition of metastasis and growth of breast cancer by co-delivery of twist shRNA and paclitaxel using pluronic P85-PEI/TPGS complex nanoparticles".BIOMATERIALS 34.5(2013):1581-1590.