Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery

doi:10.1016/j.ijpharm.2007.11.041

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	Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery
	Yang, Yongxin 1,3; Xul, Zhenghong 1; Chen, Shangwei 2; Gao, Yu 1; Gu, Wangwen1 ; Chen, Lingli1 ; Pei, Luanying 3; Li, Yaping1
刊名	INTERNATIONAL JOURNAL OF PHARMACEUTICS
	2008-04-02
卷号	353 期号:1-2 页码:277-282
关键词	polyphosphazene histidine cytotoxicity gene delivery
ISSN号	0378-5173
DOI	10.1016/j.ijpharm.2007.11.041
文献子类	Article
英文摘要	Cationic polyorganophosphazene has shown the ability to deliver gene. To obtain more efficient transfection, His(Boc)-OMe bearing histidine moiety was introduced to synthesize a new derivative of cationic polyphosphazenes with another side group of 2-dimethylaniinoethylamine (DMAEA). The poly(DMAEA/His(Boc)-OMe)phosphazene (PDHP) and DNA could self-assemble into nanoparticles with a size around 110 nm and zeta potential of +15 mV at the PDHP/DNA ratio of 10:1 (w/w). The maximum transfection efficiency of PDHP/DNA self-assembled nanoparticles (PHSNs) against 293 T cells was much higher than that of poly(di-2-dimethylan-noethylamine) phosphazenes (PDAP)/DNA self-assembled nanoparticles (PASNs) and PEI 25/DNA self-assembled nanoparticles (PESNs) at the polymer/DNA ratio of 10: 1, but the cytotoxicity of PDHP assayed by MTT was much lower than that of PDAP and PEI 25. These results suggested that PDHP could be a good candidate with high transfection efficiency and low cytotoxicity for gene delivery. (c) 2007 Elsevier B.V. All rights reserved.
WOS关键词	COMPLEXES ; TRANSFECTION ; POLYLYSINE ; SYSTEMS ; CANCER ; POLYPHOSPHAZENES ; PROTONATION ; POLYPLEXES ; THERAPY ; RELEASE
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000255320000032
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272942]
专题	药物制剂研究中心
通讯作者	Li, Yaping
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China; 2.Testing & Anal Ctr S Yangtze Univ, Wuxi 214122, Peoples R China 3.Fudan Univ, Sch Pharm, Dept Pharmaceut, Shanghai 200032, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Yongxin,Xul, Zhenghong,Chen, Shangwei,et al. Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2008,353(1-2):277-282.
APA	Yang, Yongxin.,Xul, Zhenghong.,Chen, Shangwei.,Gao, Yu.,Gu, Wangwen.,...&Li, Yaping.(2008).Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery.INTERNATIONAL JOURNAL OF PHARMACEUTICS,353(1-2),277-282.
MLA	Yang, Yongxin,et al."Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery".INTERNATIONAL JOURNAL OF PHARMACEUTICS 353.1-2(2008):277-282.