In vitro study on gene transfection efficiency of hyaluronic acid modified core-shell liponanoparticles in human retinal pigment epithelium cells

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	In vitro study on gene transfection efficiency of hyaluronic acid modified core-shell liponanoparticles in human retinal pigment epithelium cells
	Zhao Yanan 2; Gan Li 1; Wang Jing 2; Chen Xi 2; Jia Zheng 3; Gan Yong1 ; Liu Jianping 2
刊名	Acta Pharmaceutica Sinica
	2014
卷号	49 期号:5 页码:711-717
关键词	hyaluronic acid core-shell nanoparticle ARPE-19 gene delivery system
ISSN号	0513-4870
其他题名	透明质酸修饰的核壳纳米粒对视网膜色素上皮细胞基因转染效率的研究
文献子类	Article
英文摘要	The aim of this study is to prepare hyaluronic acid (HA) modified core-shell liponanoparticles (pHA-LCS-NPs) as gene delivery system and investigate its gene transfection efficiency in human retinal pigment epithelium (ARPE-19) cells in vitro. The pHA-LCS-NPs was prepared by firstly hydrating dry lipid film with CS-NPs suspension to get LCS-NPs, then modifying the lipid bilayer with HA by amidation reaction between HA and dioleoyl phosphatidylethanolamine (DOPE). Its morphology, particle size and zeta potential were investigated. XTT assay was used to evaluate the cell safety of different vectors in vitro. The gene transfection efficiency of pHA-LCS-NPs modified with different contents of HA was investigated in ARPE-19 cells with green fluorescent protein (pEGFP) as the reporter gene. The results showed that the obtained pHA-LCS-NPs exhibited a clear core-shell structure with the average particles size of (214.9 7.2) nm and zeta potential of (?35 3.7) mV. The 24 h cumulative release of gene from pHA-LCS-NPs was less than 30%. After 48 h incubation, gene transfection efficiency of pHA-LCS-NPs/pEGFP was 1.81 times and 3.75 times higher than that of CS-NPs/pEGFP and naked pEGFP, respectively. Also no obvious cytotoxicity was observed on pHA-LCS-NPs. It suggested that the pHA-LCS-NPs might be promising non-viral gene delivery systems with high efficiency and low cytotoxicity.
资助项目	国家自然科学基金资助项目[00000000] ; 上海市自然科学基金资助项目[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:5130409
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269339]
专题	药物制剂研究中心
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.China Pharmaceutical University, Nanjing, Jiangsu 210009, China.; 3.Tangshan Vocational & Technical College, Tangshan, Hebei 063000, China.
推荐引用方式 GB/T 7714	Zhao Yanan,Gan Li,Wang Jing,et al. In vitro study on gene transfection efficiency of hyaluronic acid modified core-shell liponanoparticles in human retinal pigment epithelium cells[J]. Acta Pharmaceutica Sinica,2014,49(5):711-717.
APA	Zhao Yanan.,Gan Li.,Wang Jing.,Chen Xi.,Jia Zheng.,...&Liu Jianping.(2014).In vitro study on gene transfection efficiency of hyaluronic acid modified core-shell liponanoparticles in human retinal pigment epithelium cells.Acta Pharmaceutica Sinica,49(5),711-717.
MLA	Zhao Yanan,et al."In vitro study on gene transfection efficiency of hyaluronic acid modified core-shell liponanoparticles in human retinal pigment epithelium cells".Acta Pharmaceutica Sinica 49.5(2014):711-717.