Determination of candesartan in rat plasma by HPLC-UV method and study on the gastrointestinal absorption

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	Determination of candesartan in rat plasma by HPLC-UV method and study on the gastrointestinal absorption
	GAO Fang 1; ZHANG Zhiwen2 ; LIU Li 1; JIANG Shijun 2; ZHAO Chunjie 1
刊名	Journal of Shenyang Pharmaceutical University
	2011
卷号	28 期号:6 页码:453-457
关键词	candesartan cilexetil candesartan HPLC plasma gastrointestinal absorption
ISSN号	1006-2858
其他题名	HPLC-UV法测定大鼠血浆中坎地沙坦的质量浓度及胃肠道吸收的研究
文献子类	Article
英文摘要	Objective To establish a sensitive and convenient HPLC method for the determination of the plasma concentration of candesartan and investigate the gastrointestinal absorption of candesartan cilexetil loaded solid lipid nanoparticles(SLNs)in rats.Methods The determination was performed on HPLC system with Eclipse XDB-C18 column(150 mm4.6 mm,5 mum).The mobile phase consisted of water(0.1%phosphoric acid and 0.1% triethylamine,pH 2.7)and acetonitrile(V∶ V=42∶ 58)at a flow rate of 1.0 mL · min-1,and the detection wavelenth was set at 254 nm.EXP3174 was used as the internal standard.The absorption of CSLNs in stomach and intestine was performed by intragastric and intraduodenal administration,respectively,in comparison with that of free drug suspension.Results The standard regression equations of area ratio(A)to concentration(rho)were as follows:A=7.028rho+4.20010-2,r=0.999 8(rho=0.01-1 mg · L-1);A=0.237rho+1.00*10-3,r=0.999 2(rho=0.3-30 mg · L-1),and the lower limit of quantification(LLOQ)was 10 mug · L-1.The RSDs of intra-and inter-day were less than 10.0%,and the extraction recovery was more than 75%.The validated method was successfully applied to the comparative absorption of stomach and intestine after administration of SLN and free drug suspension.It was found that the absorption of SLNs was remarkably enhanced in stomach and intestine compared with that of free drug suspension.Conclusions The HPLC method is easy,sensitive and reliable for the determination of candesartan in rat plasma.SLNs are potential for enhancing the gastrointestinal absorption of insoluble candesartan cilexetil in rats.
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:4228292
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267882]
专题	药物制剂研究中心
作者单位	1.School of Pharmacy,Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.; 2.Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	GAO Fang,ZHANG Zhiwen,LIU Li,et al. Determination of candesartan in rat plasma by HPLC-UV method and study on the gastrointestinal absorption[J]. Journal of Shenyang Pharmaceutical University,2011,28(6):453-457.
APA	GAO Fang,ZHANG Zhiwen,LIU Li,JIANG Shijun,&ZHAO Chunjie.(2011).Determination of candesartan in rat plasma by HPLC-UV method and study on the gastrointestinal absorption.Journal of Shenyang Pharmaceutical University,28(6),453-457.
MLA	GAO Fang,et al."Determination of candesartan in rat plasma by HPLC-UV method and study on the gastrointestinal absorption".Journal of Shenyang Pharmaceutical University 28.6(2011):453-457.