Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

doi:10.1016/j.arabjc.2015.06.037

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物制剂研究中心

	Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi
	Singh, Vikramjeet 1,2,3; Nand, Amita 1,2; Sarita 4; Zhang, Jiwen3 ; Zhu, Jingsong 1,2
刊名	Arabian Journal of Chemistry
	2015-04-07
ISSN号	18785352
DOI	10.1016/j.arabjc.2015.06.037
文献子类	Article
英文摘要	Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), α-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this comparative study will surely help the researchers to further upgrade these surfaces and especially SIP to make it more universal for biosensor microarray applications including biomarker discovery in high throughput format.
语种	英语
出版者	Elsevier B.V.
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266982]
专题	药物制剂研究中心
通讯作者	Singh, Vikramjeet
作者单位	1.National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China; 2.University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China; 3.Center for Drug Delivery System, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China; 4.NIMS University, Jaipur 303121, Rajasthan, India
推荐引用方式 GB/T 7714	Singh, Vikramjeet,Nand, Amita,Sarita,et al. Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi[J]. Arabian Journal of Chemistry,2015.
APA	Singh, Vikramjeet,Nand, Amita,Sarita,Zhang, Jiwen,&Zhu, Jingsong.(2015).Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi.Arabian Journal of Chemistry.
MLA	Singh, Vikramjeet,et al."Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi".Arabian Journal of Chemistry (2015).