Determination of arotinoid acid in human plasma by liquid chromatography-tandem mass spectrometry

doi:10.1016/j.jchromb.2009.07.017

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海药物代谢研究中心

	Determination of arotinoid acid in human plasma by liquid chromatography-tandem mass spectrometry
	Deng, Pan 1; Chen, Xiaoyan1 ; Tang, Yunbiao 3; Wang, Yongqing 2; Zhang, Hongwen 2; Zhong, Dafang1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2009-10-01
卷号	877 期号:27 页码:2983-2988
关键词	Retinoid Ro 13-7410 Arotinoid acid Arotinoid trometamol Liquid chromatography-tandem mass spectrometry Pharmacokinetics
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2009.07.017
文献子类	Article
英文摘要	Arotinoid acid (Ro 13-7410) is the third generation of synthetic retinoid, which was developed for the treatment of psoriasis and other hyperkeratotic skin disorders. The therapeutically active dose is less than 0.5 mu g/kg body weight/day. To investigate the pharmacokinetics of arotinoid acid, a sensitive and selective liquid chromatographic-tandem mass spectrometric method (LC-MS/MS) for the determination of arotinoid acid in human plasma was developed and validated. The sample processing was carried out in the dark to minimize photodegradation of the analytes. Arotinoid acid and the internal standard (IS), acitretin, were extracted from plasma samples using solid phase extraction with Oasis HLB cartridges. Chromatographic separation was achieved on a Zorbax Extend C-18 column (150 mm x 4.6 mm, i.d., 5 mu m) using methanol:acetonitrile:5 mM ammonium acetate (48:32:20, v/v/v) as the mobile phase at a flow rate of 0.8 mL/min. The detection was carried out in multiple reaction monitoring (MRM) mode via negative electrospray ionization (ESI) interface. The lower limit of quantification (LLOQ) achieved was 37.1 pg/mL with intra-day and inter-day precision (RSD) of 8.7% and 8.5%, and accuracy (RE) of 2.0%. Inter-day and intra-day RSD for three quality control levels (QCs) across validation runs were less than 11.0% and the accuracy ranged from 1.9% to 3.3%. The validated LC-MS/MS method was applied to a phase I clinical pharmacokinetic study after a single oral administration of 40 mu g arotinoid trometamol to healthy subjects. (C) 2009 Published by Elsevier B.V.
WOS关键词	ENDOGENOUS RETINOIDS ; SYSTEMIC RETINOIDS ; BIOLOGICAL-FLUIDS ; RO 13-7410 ; DERMATOLOGY ; THERAPY ; SERUM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000270246400007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279116]
专题	上海药物代谢研究中心
通讯作者	Zhong, Dafang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Nanjing Med Univ, Affiliated Hosp 1, Nanjing 210029, Peoples R China 3.Gen Hosp Shenyang Mil Command, Shenyang 110016, Peoples R China;
推荐引用方式 GB/T 7714	Deng, Pan,Chen, Xiaoyan,Tang, Yunbiao,et al. Determination of arotinoid acid in human plasma by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(27):2983-2988.
APA	Deng, Pan,Chen, Xiaoyan,Tang, Yunbiao,Wang, Yongqing,Zhang, Hongwen,&Zhong, Dafang.(2009).Determination of arotinoid acid in human plasma by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,877(27),2983-2988.
MLA	Deng, Pan,et al."Determination of arotinoid acid in human plasma by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.27(2009):2983-2988.