Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma

doi:10.1016/j.jchromb.2012.08.019

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	Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma
	Chen, Meixia; Xia, Yu; Ma, Zhiyu; Li, Liang; Zhong, Dafang; Chen, Xiaoyan
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2012-10-01
卷号	906 页码:85-90
关键词	Chiral LC-MS/MS Pantoprazole Stereoselective pharmacokinetics
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2012.08.019
文献子类	Article
英文摘要	Pantoprazole (PAN), a selective proton pump inhibitor, is used clinically as a racemic mixture for the treatment of acid-related gastrointestinal disorders. To investigate its stereoselective pharmacokinetics, a chiral liquid chromatography-tandem mass spectrometry method was developed and validated to determine the pantoprazole enantiomers in dog plasma. After liquid-liquid extraction, a baseline resolution of enantiomers was achieved on an ovomucoid column using the mobile phase of methanol:acetonitrile:10 mM ammonium formate (pH 7) (10.4:2.6:87, v/v/v) at 30 degrees C within 10 min. Stable isotopically labeled (+)-d(3)-pantoprazole and (-)-d(3)-pantoprazole were used as internal standards. Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode via positive atmospheric pressure chemical ionization. The method was linear in the concentration range of 20.0-10,000 ng/mL for each enantiomer using 25 mu L of dog plasma. The lower limit of quantification (LLOQ) for each enantiomer was 20.0 ng/mL. Intra- and inter-day precision ranged from 3.2% to 10.3% for (+)-pantoprazole and 3.7-10.0% for (-)-pantoprazole. Accuracy varied from -1.4% to -0.2% for (+)-pantoprazole and -1.6% to 0.8% for (-)-pantoprazole. The validated method was applied successfully for stereoselective pharmacokinetic studies of racemic pantoprazole. (C) 2012 Elsevier B.V. All rights reserved.
资助项目	National Basic Research Program of China[2009CB930300]
WOS关键词	PROTON PUMP INHIBITOR ; STEREOSELECTIVE PHARMACOKINETICS ; POOR METABOLIZERS ; STATIONARY-PHASE ; S-MEPHENYTOIN ; LC-MS/MS ; ENANTIOMERS ; RATS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000309440600012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277922]
专题	上海药物代谢研究中心
通讯作者	Chen, Xiaoyan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Chen, Meixia,Xia, Yu,Ma, Zhiyu,et al. Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,906:85-90.
APA	Chen, Meixia,Xia, Yu,Ma, Zhiyu,Li, Liang,Zhong, Dafang,&Chen, Xiaoyan.(2012).Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,906,85-90.
MLA	Chen, Meixia,et al."Validation of a chiral liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole in dog plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 906(2012):85-90.