High-sensitivity liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sodium picosulfate and its three major metabolites in human plasma

doi:10.1016/j.jchromb.2012.12.007

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	High-sensitivity liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sodium picosulfate and its three major metabolites in human plasma
	Yang, Yong; Yu, Jinghua; Lu, Youming; Xia, Yu; Zhong, Dafang; Chen, Xiaoyan
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2013-02-01
卷号	915 页码:1-7
关键词	Sodium picosulfate Glucuronide conjugate Sulfate conjugate LC-MS/MS Pharmacokinetics
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2012.12.007
文献子类	Article
英文摘要	Sodium picosulfate (PICO-Na) is a member of the polyphenolic group of stimulant laxatives. Its major metabolites in humans are its active aglycone BHPM (bis-(p-hydroxyphenyl)-pyridyl-2-methane), the monoglucuronide (M1) and the monosulfate (M2) of BHPM. A sensitive, specific and rapid liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of picosulfate (PICO) and its three major metabolites in human plasma to investigate the pharmacokinetics of PICO and its major metabolites. Following protein precipitation with acetonitrile, chromatographic separation was achieved on a Luna 5u C-18 (2) column using gradient elution starting with 10% of 10 mM ammonium acetate followed by increasing percentages of acetonitrile to eliminate interferences due to in-source conversion of the conjugated metabolites. Detection was performed on a tandem mass spectrometer equipped with an electrospray ionization source operated in the positive mode, using the transitions of m/z 438.1 -> m/z 278.1 for PICO, m/z 278.1 -> m/z 184.2 for BHPM, m/z 454.1 -> m/z 184.2 for M1, and m/z 358.1 -> m/z 184.2 summed with m/z 358.1 -> m/z 278.1 for M2. Deuterium labeled compounds of the analytes were used as the internal standard, two of which, M1-d(12) and M2-d(12), were synthesized in-house. The method was validated in concentration ranges of 0.150-40.0 ng/mL for PICO and M2, 0.600-160 ng/mL for BHPM, and 0.045-12.0 ng/mL for M1 with acceptable accuracy and precision. The method was successfully applied to characterize the pharmacokinetic profiles of PICO and its metabolites in healthy volunteers after a single oral administration of 5 mg PICO-Na. (C) 2013 Elsevier B.V. All rights reserved.
WOS关键词	BISACODYL ; EXCRETION ; GLUCURONIDE ; MS/MS ; RAT
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000314559800001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277746]
专题	上海药物代谢研究中心
通讯作者	Chen, Xiaoyan
作者单位	Chinese Acad Sci, Ctr Drug Metab & Pharmacokinet, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yong,Yu, Jinghua,Lu, Youming,et al. High-sensitivity liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sodium picosulfate and its three major metabolites in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,915:1-7.
APA	Yang, Yong,Yu, Jinghua,Lu, Youming,Xia, Yu,Zhong, Dafang,&Chen, Xiaoyan.(2013).High-sensitivity liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sodium picosulfate and its three major metabolites in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,915,1-7.
MLA	Yang, Yong,et al."High-sensitivity liquid chromatography-tandem mass spectrometry method for the simultaneous determination of sodium picosulfate and its three major metabolites in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 915(2013):1-7.