A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells

doi:10.1016/j.jchromb.2014.01.021

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	A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells
	Tu, Xiaowei; Lu, Youming; Zhong, Dafang; Zhang, Yifan; Chen, Xiaoyan
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2014-08-01
卷号	964 页码:202-207
关键词	Clofarabine Clofarabine triphosphate Liquid chromatography-tandem mass spectrometry Peripheral blood mononuclear cells
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2014.01.021
文献子类	Article
英文摘要	Clofarabine triphosphate is an intracellular active metabolite of clofarabine. In the present study, we developed and validated a rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from blood using the Ficoll gradient centrifugation method. Chromatographic separation was performed on a CN column using an isocratic mobile phase comprising acetonitrile/5 mM ammonium acetate with 0.001% ammonium hydroxide (20/80, v/v) at a flow rate of 0.60 mL/min. Detection was carried out by MS/MS in the multiple reaction monitoring mode using a negative electrospray ionization interface. The method was validated in concentration ranges of 1.25-100 ng/10(7) cells with acceptable accuracy and precision using 50 mu L of cell extract. Clofarabine triphosphate was stable in a series of stability studies with bench-top, auto-sampler, and repeated freeze-thaw cycles. The validated method was successfully used to measure the concentrations of clofarabine triphosphate in PBMCs from cancer patients treated with clofarabine (C) 2014 Elsevier B.V. All rights reserved.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; NUCLEOSIDE TRANSPORTERS ; LEUKEMIA ; QUANTITATION ; CANCER ; ANALOG ; DRUGS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000338977000021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276975]
专题	上海药物代谢研究中心
通讯作者	Chen, Xiaoyan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Tu, Xiaowei,Lu, Youming,Zhong, Dafang,et al. A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,964:202-207.
APA	Tu, Xiaowei,Lu, Youming,Zhong, Dafang,Zhang, Yifan,&Chen, Xiaoyan.(2014).A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,964,202-207.
MLA	Tu, Xiaowei,et al."A sensitive LC-MS/MS method for quantifying clofarabine triphosphate concentrations in human peripheral blood mononuclear cells".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 964(2014):202-207.