Development of a UHPLC-MS/MS method for the determination of plasma histamine in various mammalian species

doi:10.1016/j.jchromb.2014.08.043

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	Development of a UHPLC-MS/MS method for the determination of plasma histamine in various mammalian species
	Liu, Jia; Wang, Lei; Hu, Wenjuan; Chen, Xiaoyan; Zhong, Dafang
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2014-11-15
卷号	971 页码:35-42
关键词	Histamine UPLC HILIC column LC-MS/MS Plasma concentration
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2014.08.043
文献子类	Article
英文摘要	Histamine is an important mediator of anaphylactic reactions. Although several methods have been developed to measure histamine levels, each has its limitations. In this study, we developed and validated a convenient bioanalytical method for the qualitative and quantitative determination of histamine in plasma samples from humans, beagle dogs, Sprague-Dawley rats, and imprinting control region mice. A simple plasma protein precipitation method using acetonitrile was selected, and hydrophilic interaction liquid chromatography coupled with mass spectrometry was used for sample separation and detection. Histamine was subjected to gradient elution with acetonitrile, ammonium acetate buffer, and formic acid. A mass spectrometer equipped with an electrospray ionization source was operated in the positiveion multiple reaction monitoring mode for the detection of histamine and the internal standard. The [M + H](+) transitions were m/z 112 -> 95 for histamine and m/z 116 -> 99 for d(4)-histamine, which was used as the internal standard. The lower limit of quantification was 0.2 mu g/L and the calibration range was 0.2-500 mu g/L. The overall recovery ranged from 93.6% to 102.8%. The intra- and inter-run precision and accuracy were <15% for plasma samples from all four species. The method was validated by measuring the plasma histamine concentrations in five healthy human volunteers. In conclusion, we have developed and validated a novel bioanalytical method for the quantification of histamine levels in plasma samples from various mammalian species. (C) 2014 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81173117]
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; DERIVATIZATION-CAPILLARY ELECTROCHROMATOGRAPHY ; FLUORESCENCE DETECTION ; MASS-SPECTROMETRY ; BIOGENIC-AMINES ; PRECOLUMN DERIVATIZATION ; SENSITIVE DETERMINATION ; WHOLE-BLOOD ; KU812 CELLS ; METABOLITES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000344211100006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276835]
专题	上海药物代谢研究中心
通讯作者	Zhong, Dafang
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jia,Wang, Lei,Hu, Wenjuan,et al. Development of a UHPLC-MS/MS method for the determination of plasma histamine in various mammalian species[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,971:35-42.
APA	Liu, Jia,Wang, Lei,Hu, Wenjuan,Chen, Xiaoyan,&Zhong, Dafang.(2014).Development of a UHPLC-MS/MS method for the determination of plasma histamine in various mammalian species.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,971,35-42.
MLA	Liu, Jia,et al."Development of a UHPLC-MS/MS method for the determination of plasma histamine in various mammalian species".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 971(2014):35-42.