High-throughput salting-out-assisted liquid-liquid extraction for the simultaneous determination of atorvastatin, ortho-hydroxyatorvastatin, and para-hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry

doi:10.1002/jssc.201401227

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海药物代谢研究中心

	High-throughput salting-out-assisted liquid-liquid extraction for the simultaneous determination of atorvastatin, ortho-hydroxyatorvastatin, and para-hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry
	Yang, Yong; Xu, Qiufang; Zhou, Lei; Zhong, Dafang; Chen, Xiaoyan
刊名	JOURNAL OF SEPARATION SCIENCE
	2015-03
卷号	38 期号:6 页码:1026-1034
ISSN号	1615-9306
DOI	10.1002/jssc.201401227
文献子类	Article
英文摘要	A high-throughput, specific, and rapid liquid chromatography with tandem mass spectrometry method was established and validated for the simultaneous determination of atorvastatin and its two major metabolites, ortho-hydroxyatorvastatin and para-hydroxyatorvastatin, in human plasma. A simple salting-out-assisted liquid-liquid extraction using acetonitrile and a mass-spectrometry-friendly salt, ammonium acetate, was employed to extract the analytes from human plasma. A recovery of more than 81% for all analytes was achieved in 1 min extraction time. Chromatographic separation was performed on a Kinetex XB C-18 column utilizing a gradient elution starting with a 60% of water solution (1% formic acid), followed by increasing percentages of acetonitrile. Analytes were detected on a tandem mass spectrometer equipped with an electrospray ionization source that was operated in the positive mode, using the transitions of m/z 559.3 -> m/z 440.2 for atorvastatin, and m/z 575.3 -> m/z 440.2 for both ortho-and para-hydroxyatorvastatin. Deuterium-labeled compounds were used as the internal standards. The method was validated over the concentration ranges of 0.0200-15.0 ng/mL for atorvastatin and ortho-hydroxyatorvastatin, and 0.0100-2.00 ng/mL for para-hydroxyatorvastatin with acceptable accuracy and precision. It was then successfully applied in a bioequivalence study of atorvastatin.
WOS关键词	PARA-HYDROXY ATORVASTATIN ; COA REDUCTASE INHIBITOR ; SOLID-PHASE EXTRACTION ; LIQUID/LIQUID EXTRACTION ; LACTONE FORMS ; HUMAN SERUM ; MS-MS ; VALIDATION ; QUANTITATION ; METABOLITES
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000351598400019
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276618]
专题	上海药物代谢研究中心
通讯作者	Chen, Xiaoyan
作者单位	Chinese Acad Sci, Ctr Drug Metab & Pharmacokinet, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yong,Xu, Qiufang,Zhou, Lei,et al. High-throughput salting-out-assisted liquid-liquid extraction for the simultaneous determination of atorvastatin, ortho-hydroxyatorvastatin, and para-hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(6):1026-1034.
APA	Yang, Yong,Xu, Qiufang,Zhou, Lei,Zhong, Dafang,&Chen, Xiaoyan.(2015).High-throughput salting-out-assisted liquid-liquid extraction for the simultaneous determination of atorvastatin, ortho-hydroxyatorvastatin, and para-hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,38(6),1026-1034.
MLA	Yang, Yong,et al."High-throughput salting-out-assisted liquid-liquid extraction for the simultaneous determination of atorvastatin, ortho-hydroxyatorvastatin, and para-hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 38.6(2015):1026-1034.