Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection

doi:10.1016/j.jchromb.2007.04.025

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	Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection
	Zhou, Maojin ; Chen, Xiaoyan; Zhong, Dafang
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2007-07-01
卷号	854 期号:1-2 页码:219-223
关键词	trans-resveratrol-3-O-glucoside trans-resveratrol-3-O-glucuronide trans-resveratrol
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2007.04.025
文献子类	Article
英文摘要	A sensitive and selective high- performance liquid chromatographic method was developed for simultaneous determination of trans-resveratrol-3-O-glucoside (TRG) and its metabolites, trans-resveratrol-3-O-glucuronide (TRN) and trans-resveratrol (TR) in rat plasma. The plasma proteins were precipitated with acetonitrile and supernatant was evaporated to dryness. The analytes and internal standard baicalin were chromatographed on a Cls column. The mobile phase consisted of 25% acetonitrile and 75% H2O adjusted with formic acid to pH 3.5. The flow-rate was 1.0 ml/min and ultraviolet detection was set at 320 nm. Standard curves were linear over the concentration range of 0.04-40 mu g/ml for TRG and TRN, and 0.04-10 mu g/ml for TR, respectively. The precision, expressed as the intra-day R.S.D. and inter-day R.S.D., was below 9.3% for TRG, TRN and TR. The accuracy, expressed as the relative error (RE) was within 7.4% for all analytes. The mean recoveries of TRG, TRN, TR and I.S. were 93.6%, 93.1%. 91.0% and 87.9%, respectively. This method was successfully applied to a pharmacokinetic study of TRG after an oral dose of 150 mg/kg to Wistar rats. (C) 2007 Elsevier B.V. All rights reserved.
WOS关键词	TRANS-RESVERATROL ; STILBENE COMPONENTS ; SMALL-INTESTINE ; POLYPHENOLS ; ABSORPTION ; ROOTS ; WINE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000248161900032
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273212]
专题	上海药物代谢研究中心
通讯作者	Zhong, Dafang
作者单位	1.Shenyang Pharmaceut Univ, Lab Drug Metab & Pharmacokinet, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Maojin,Chen, Xiaoyan,Zhong, Dafang. Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,854(1-2):219-223.
APA	Zhou, Maojin,Chen, Xiaoyan,&Zhong, Dafang.(2007).Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,854(1-2),219-223.
MLA	Zhou, Maojin,et al."Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 854.1-2(2007):219-223.