Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR

doi:10.1038/embor.2010.96

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR
	Chen, Hao 1,5; Yi, Chengqi 2,3; Zhang, Jin 1,5; Zhang, Wenru 4; Ge, Zhiyun 1,5; Yang, Cai-Guang4 ; He, Chuan 2,3
刊名	EMBO REPORTS
	2010-09
卷号	11 期号:9 页码:685-690
关键词	antibiotic resistance regulation oxidative stress Pseudomonas aeruginosa redox sensing thiol oxidation
ISSN号	1469-221X
DOI	10.1038/embor.2010.96
文献子类	Article
英文摘要	MexR functions as the primary regulator of the mexAB-oprM multidrug efflux expression in Pseudomonas aeruginosa. It has been shown that MexR senses oxidative stress by interprotomer disulphide bond formation between redox-active cysteines. This oxidation induces MexR to dissociate from the promoter DNA, thus activating the transcriptional expression of efflux pump genes. In this study, we present the crystal structure of MexR in its oxidized form at a resolution of 2.1 angstrom. This crystal structure reveals the mechanism by which oxidative signal allosterically derepresses the MexR-controlled transcription activation.
资助项目	National Institutes of Health (NIAID)[R01 AI074658] ; National Natural Science Foundation of China (NSFC)[20721002] ; Chinese Academy of Sciences[00000000] ; NSFC[90913010] ; Key Project of Chinese National Programs for Fundamental Research and Development[2009CB918502] ; National Science and Technology Major Project[2009ZX09301-001]
WOS关键词	MULTIDRUG EFFLUX OPERON ; PSEUDOMONAS-AERUGINOSA ; CRYSTAL-STRUCTURE ; REPRESSOR ; PUMPS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000281467200012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278785]
专题	药理学第三研究室药物靶标结构与功能中心
通讯作者	Chen, Hao
作者单位	1.Nanjing Univ, State Key Lab Coordinat Chem, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China; 2.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 5.Nanjing Univ, Inst Coordinat Chem, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China;
推荐引用方式 GB/T 7714	Chen, Hao,Yi, Chengqi,Zhang, Jin,et al. Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR[J]. EMBO REPORTS,2010,11(9):685-690.
APA	Chen, Hao.,Yi, Chengqi.,Zhang, Jin.,Zhang, Wenru.,Ge, Zhiyun.,...&He, Chuan.(2010).Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR.EMBO REPORTS,11(9),685-690.
MLA	Chen, Hao,et al."Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR".EMBO REPORTS 11.9(2010):685-690.