Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative | |
Xu, Yuxia; Dong, Qun; Qiu, Hong; Cong, Renhuai; Ding, Kan![]() | |
刊名 | BIOMACROMOLECULES
![]() |
2010-10 | |
卷号 | 11期号:10页码:2558-2566 |
ISSN号 | 1525-7797 |
DOI | 10.1021/bm100402n |
文献子类 | Article |
英文摘要 | A water-soluble polysaccharide, PGAW1, with an average molecular mass of 9.2 kDa determined by high performance gel permeation chromatography (HPGPC), was isolated from radix of Platycodon grandifloram. Monosaccharide composition analysis indicated that PGAW1 contains Ara and Gal in the molar ratio of 1.42:1.0. Using methylation analysis, partial hydrolysis, endo-1,4-beta-D-galactanase digestion. NMR and ESI-MS. PGAW1 was determined to possess a backbone consisting of 1,4- and 1,6-linked galactopyranosyl residues, with branches attached to O-3 of 1,6-linked galactose residues. By the chlorosulfonic acid-pyridine method we produced a sulfated derivative of PGAW1, Sul-wl, with a substitution degree of 1.52. The substitution was to O-6 on 1,4-linked Gal residues according to the C-13 NMR spectra, Bioactivity test showed that Sul-wl could inhibit tube formation by human microvascular endothelial cells (HMEC) in a dose-dependent manner. |
资助项目 | National Science Foundation[30870551] ; Key New Drug Creation and Manufacturing Program[2009ZX09501-011] ; Key New Drug Creation and Manufacturing Program[2009ZX09103-071] ; Key New Drug Creation and Manufacturing Program[2009ZX09301-001] |
WOS关键词 | ANGIOGENESIS INHIBITORS ; D-GLUCAN ; POLYSACCHARIDES ; CELLS ; RADIX ; RATS |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000282840200004 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/278755] ![]() |
专题 | 药理学第三研究室 |
通讯作者 | Dong, Qun |
作者单位 | Chinese Acad Sci & Infinitus, Shanghai Inst Materia Med, Joint Lab Res Chinese Herbal Polysaccharides, Glycobiol & Glycochem Lab, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Yuxia,Dong, Qun,Qiu, Hong,et al. Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative[J]. BIOMACROMOLECULES,2010,11(10):2558-2566. |
APA | Xu, Yuxia,Dong, Qun,Qiu, Hong,Cong, Renhuai,&Ding, Kan.(2010).Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative.BIOMACROMOLECULES,11(10),2558-2566. |
MLA | Xu, Yuxia,et al."Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative".BIOMACROMOLECULES 11.10(2010):2558-2566. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论