Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative

doi:10.1021/bm100402n

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative
	Xu, Yuxia; Dong, Qun; Qiu, Hong; Cong, Renhuai; Ding, Kan
刊名	BIOMACROMOLECULES
	2010-10
卷号	11 期号:10 页码:2558-2566
ISSN号	1525-7797
DOI	10.1021/bm100402n
文献子类	Article
英文摘要	A water-soluble polysaccharide, PGAW1, with an average molecular mass of 9.2 kDa determined by high performance gel permeation chromatography (HPGPC), was isolated from radix of Platycodon grandifloram. Monosaccharide composition analysis indicated that PGAW1 contains Ara and Gal in the molar ratio of 1.42:1.0. Using methylation analysis, partial hydrolysis, endo-1,4-beta-D-galactanase digestion. NMR and ESI-MS. PGAW1 was determined to possess a backbone consisting of 1,4- and 1,6-linked galactopyranosyl residues, with branches attached to O-3 of 1,6-linked galactose residues. By the chlorosulfonic acid-pyridine method we produced a sulfated derivative of PGAW1, Sul-wl, with a substitution degree of 1.52. The substitution was to O-6 on 1,4-linked Gal residues according to the C-13 NMR spectra, Bioactivity test showed that Sul-wl could inhibit tube formation by human microvascular endothelial cells (HMEC) in a dose-dependent manner.
资助项目	National Science Foundation[30870551] ; Key New Drug Creation and Manufacturing Program[2009ZX09501-011] ; Key New Drug Creation and Manufacturing Program[2009ZX09103-071] ; Key New Drug Creation and Manufacturing Program[2009ZX09301-001]
WOS关键词	ANGIOGENESIS INHIBITORS ; D-GLUCAN ; POLYSACCHARIDES ; CELLS ; RADIX ; RATS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000282840200004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278755]
专题	药理学第三研究室
通讯作者	Dong, Qun
作者单位	Chinese Acad Sci & Infinitus, Shanghai Inst Materia Med, Joint Lab Res Chinese Herbal Polysaccharides, Glycobiol & Glycochem Lab, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Xu, Yuxia,Dong, Qun,Qiu, Hong,et al. Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative[J]. BIOMACROMOLECULES,2010,11(10):2558-2566.
APA	Xu, Yuxia,Dong, Qun,Qiu, Hong,Cong, Renhuai,&Ding, Kan.(2010).Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative.BIOMACROMOLECULES,11(10),2558-2566.
MLA	Xu, Yuxia,et al."Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative".BIOMACROMOLECULES 11.10(2010):2558-2566.