Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities

doi:10.1016/j.carbpol.2011.06.029

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities
	Chen, Xia 2; Cao, Dianxiu 2; Zhou, Ling 2; Jin, Hongying 1; Dong, Qun 2; Yao, Jian 2; Ding, Kan2
刊名	CARBOHYDRATE POLYMERS
	2011-08-30
卷号	86 期号:3 页码:1300-1305
关键词	Gastrodia elata Bl. Poly-/oligosaccharide Anti-cancer Pancreatic cancer
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2011.06.029
文献子类	Article
英文摘要	To discover anti-pancreatic cancer cells growth inhibitor, a new water-soluble glucan, WTMA, was isolated from the rhizome of Gastrodia elate Bl. The mean molecular weight of the polysaccharide was estimated to be 7.0 x 10(5) Da by high performance gel permeation chromatography (HPGPC). The structure of WTMA was characterized by GC, GC-MS, NMR and MALDI-TOF. WTMA was deduced as an alpha-(1 --> 4)-glucan with alpha-(1 --> 4) linked branches attached to O-6 at branch points. Bioactivity tests in vitro indicated that WTMA and its partial acid hydrolysis-derived mixture product, WTMA-AD-O, could significantly inhibit the proliferation of pancreatic cancer cells. However, WTMA-DE, the degraded polysaccharide without branch chain by isoamylase, did not inhibit the proliferation of pancreatic cancer cells. Thus side chain on WTMA was deduced to be required for the anti-cancer cell activity. Further study demonstrated that WTMA and all of its hydrolysis products had no inhibition effect on liver LO2 cells. (C) 2011 Elsevier Ltd. All rights reserved.
资助项目	Chinese Academy of Sciences, China[00000000] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-011] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09103-071]
WOS关键词	GRIFOLA-FRONDOSA ; ANTITUMOR-ACTIVITY ; SODIUM-HYDROXIDE ; LENTINUS-EDODES ; 5-FLUOROURACIL ; CONFORMATION ; BODIES ; ALPHA
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000295308500028
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278428]
专题	药理学第三研究室
通讯作者	Ding, Kan
作者单位	1.Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Chen, Xia,Cao, Dianxiu,Zhou, Ling,et al. Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities[J]. CARBOHYDRATE POLYMERS,2011,86(3):1300-1305.
APA	Chen, Xia.,Cao, Dianxiu.,Zhou, Ling.,Jin, Hongying.,Dong, Qun.,...&Ding, Kan.(2011).Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities.CARBOHYDRATE POLYMERS,86(3),1300-1305.
MLA	Chen, Xia,et al."Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities".CARBOHYDRATE POLYMERS 86.3(2011):1300-1305.