PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells

doi:10.1002/jcb.24147

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells
	Zhang, Xiaodong 1; Yan, Guirui 1; Ji, Jun 1,2; Wu, Jingwei 1; Sun, Xiaoyun 1; Shen, Jingshan1 ; Jiang, Hualiang1 ; Wang, Heyao1
刊名	JOURNAL OF CELLULAR BIOCHEMISTRY
	2012-08
卷号	113 期号:8 页码:2738-2743
关键词	MELANIN SYNTHESIS PDE5 INHIBITOR PKG TYROSINASE
ISSN号	0730-2312
DOI	10.1002/jcb.24147
文献子类	Article
英文摘要	PDE inhibitors could increase cellular cGMP levels and are used to treat erectile dysfunction as well as pulmonary arterial hypertension. cGMP production was reported to be necessary for UVB-induced melanin synthesis, however, the effect of PDE5 inhibitor on melanin synthesis has not been examined. We found that PDE5 inhibitor (sildenafil or vardenafil) and the cGMP analog 8-CPT-cGMP stimulated CREB phosphorylation, leading to increased tyrosinase expression and melanin synthesis, which was counteracted by KT5823, a selective cGMP-dependent protein kinase (PKG) inhibitor. However, KT5823 did not affect cAMP-elevating agent-mediated melanin synthesis, indicating that KT5823 selectively inhibited cGMP-induced melanin synthesis. This is the first study to find that PDE5 inhibitor can promote melanin synthesis and reveal that PKG-dependent CREB phosphorylation and tyrosinase expression is involved in cGMP-induced melanin synthesis. Our results suggest that PDE5 inhibitor may be beneficial for the treatment of hypopigmentation diseases. J. Cell. Biochem. 113: 27382743, 2012. (c) 2012 Wiley Periodicals, Inc.
资助项目	Shanghai Science and Technology Innovation Program[08431900800] ; National Natural Science Foundation of China[81072681] ; National Natural Science Foundation of China[20972174] ; National Science and Technology Major[2009ZX09301-001] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001]
WOS关键词	TRANSCRIPTION FACTOR ; HUMAN MELANOCYTES ; KINASE ACTIVATION ; NITRIC-OXIDE ; MELANOGENESIS ; SKIN ; CREB ; PIGMENTATION ; DISORDERS ; NETWORK
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000305334900021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278007]
专题	药理学第三研究室
通讯作者	Wang, Heyao
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaodong,Yan, Guirui,Ji, Jun,et al. PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2012,113(8):2738-2743.
APA	Zhang, Xiaodong.,Yan, Guirui.,Ji, Jun.,Wu, Jingwei.,Sun, Xiaoyun.,...&Wang, Heyao.(2012).PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells.JOURNAL OF CELLULAR BIOCHEMISTRY,113(8),2738-2743.
MLA	Zhang, Xiaodong,et al."PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells".JOURNAL OF CELLULAR BIOCHEMISTRY 113.8(2012):2738-2743.