Structure of a beta-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-kappa B signaling

doi:10.1007/s10719-012-9416-z

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structure of a beta-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-kappa B signaling
	Fang, Jianping; Wang, Ying; Lv, Xiaofen; Shen, Xiaokun; Ni, Xinyan; Ding, Kan
刊名	GLYCOCONJUGATE JOURNAL
	2012-08
卷号	29 期号:5-6 页码:365-377
关键词	beta-glucan Grifola frondosa Biological response modifier Dectin-1
ISSN号	0282-0080
DOI	10.1007/s10719-012-9416-z
文献子类	Article
英文摘要	A soluble homogeneous beta-glucan, GFPBW1, with a molecular mass of 300 kDa was purified from the fraction of the fruit bodies of Grifola frondosa extracted with 5 % NaOH. Using various methods, such as infrared spectroscopy, NMR, methylation and monosaccharide composition analysis, its structure was determined to be a beta-D-(1-3)-linked glucan backbone with a single beta-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue. It induced TNF-alpha and IL-6 production and the activation of Syk and NF-kappa B signaling in resident peritoneal macrophages from ICR mice, which could be significantly inhibited by the blocking reagent laminarin. A competitive phagocytosis assay with FITC-zymosan indicated that GFPBW1 could bind to DC-associated C-type lectin 1 (Dectin-1). The TNF-alpha secretion and activation of Syk/NF-kappa B signaling triggered by GFPBW1 were enhanced in RAW264.7 cells overexpressing wild but not mutant (Delta 38 and Y15S) Dectin-1. Furthermore, GFPBW1 potentiated the Concanavalin A-induced proliferative response of splenocytes and inhibited Sarcoma-180 growth allografted in ICR mice but not in immunodeficient BALB/c nu/nu mice. These results suggested that the antitumor activity of GFPBW1 was partially associated with the activation of macrophages via the Dectin-1/Syk/NF-kappa B signaling pathway. This molecule could be a promising biological response modifier with clear application for antitumor therapies.
资助项目	New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; National Science Fund for Distinguished Young Scholars[81125025] ; funds for Industry-University-Research Institution Alliance in Guangdong Province, China[2010A090200041]
WOS关键词	CULTURED FRUIT BODIES ; GANODERMA-LUCIDUM ; IMMUNE-SYSTEM ; HOST-DEFENSE ; DECTIN-1 ; MACROPHAGES ; FUNGAL ; POLYSACCHARIDE ; RECOGNITION ; INDUCTION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000307763900014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277998]
专题	药理学第三研究室
通讯作者	Ding, Kan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Fang, Jianping,Wang, Ying,Lv, Xiaofen,et al. Structure of a beta-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-kappa B signaling[J]. GLYCOCONJUGATE JOURNAL,2012,29(5-6):365-377.
APA	Fang, Jianping,Wang, Ying,Lv, Xiaofen,Shen, Xiaokun,Ni, Xinyan,&Ding, Kan.(2012).Structure of a beta-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-kappa B signaling.GLYCOCONJUGATE JOURNAL,29(5-6),365-377.
MLA	Fang, Jianping,et al."Structure of a beta-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-kappa B signaling".GLYCOCONJUGATE JOURNAL 29.5-6(2012):365-377.