Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome

doi:10.1371/journal.pone.0065381

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	Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome
	Liu, Ping 2,3; Wang, Yuan-yuan 2; Qi, Xin 2; Gu, QianQun 2; Geng, Meiyu1 ; Li, Jing2
刊名	PLOS ONE
	2013-06-14
卷号	8 期号:6
ISSN号	1932-6203
DOI	10.1371/journal.pone.0065381
文献子类	Article
英文摘要	Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs.
资助项目	National High-tech R&D Program of China[2011AA09070104] ; Program for Changjiang Scholars and Innovative Research Team in University[IRT0944]
WOS关键词	CYCLOPRODIGIOSIN HYDROCHLORIDE ; H+/CL-SYMPORTER ; HL-60 CELLS ; H+-ATPASE ; IN-VITRO ; PRODIGIOSIN ; LINES ; DIFFERENTIATION ; PROLIFERATION ; INHIBITION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000320363300018
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277582]
专题	药理学第一研究室
通讯作者	Geng, Meiyu
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China; 3.Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Ping,Wang, Yuan-yuan,Qi, Xin,et al. Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome[J]. PLOS ONE,2013,8(6).
APA	Liu, Ping,Wang, Yuan-yuan,Qi, Xin,Gu, QianQun,Geng, Meiyu,&Li, Jing.(2013).Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome.PLOS ONE,8(6).
MLA	Liu, Ping,et al."Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome".PLOS ONE 8.6(2013).