Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells

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	Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells
	Jiang, JF; Liu, WJ; Ding, J
刊名	ACTA PHARMACOLOGICA SINICA
	2000-08
卷号	21 期号:8 页码:759-764
关键词	camptothecin apoptosis telomerase bcl-2 genes
ISSN号	0253-9756
文献子类	Article
英文摘要	AIM: To explore the regulation of telomerase activity in camptothecin induced apoptosis of human leukemia HL-60 cells. METHODS: Apoptosis of HL-60 cells was induced by camptothecin (1 mg/L) for 2, 4, and 6 h. Apoptosis was determined by agarose electrophoresis and flow cytometry analysis. To assess telomerase activity, a PCR-based telomeric repeat amplification protocol assay (TRAP) was used. RT-PCR was performed to examine the mRNA levels of hTR (human telomerase RNA component), hEST2/hTERT (human telomerase reverse transcriptase), TLP1/TP1 (telomerase association protein 1), and bcl-2 (B cell leukemia/lymphoma 2 gene) in HL-60 cells before and after camptothecin treatment. RESULTS: Telomerase activity was decreased in a time-dependent manner during the camptothecin induced apoptosis of HL-60 cells. However, no difference in expression of each telomerase subunit was detected, while expression of bcl-2 was progressively down-regulated. CONCLUSION: These lines of evidences indicated that down-regulation of the telomerase activity in HL-60 cells was closely related to camptothecin-induced apoptosis, and the telomerase activity was not blocked at the transcriptional levels of the genes of the known ribonucleoprotein (RNP) complex components. We also found that bcl-2 did not regulate the mRNA expressions of telomerase subunits directly.
WOS关键词	IMMORTAL CELLS ; COMPONENT ; TUMORS ; CANCER ; GENE
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000088658900016
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274588]
专题	药理学第一研究室
通讯作者	Ding, J
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Jiang, JF,Liu, WJ,Ding, J. Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells[J]. ACTA PHARMACOLOGICA SINICA,2000,21(8):759-764.
APA	Jiang, JF,Liu, WJ,&Ding, J.(2000).Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells.ACTA PHARMACOLOGICA SINICA,21(8),759-764.
MLA	Jiang, JF,et al."Regulation of telomerase activity in camptothecin-induced apoptosis of human leukemia HL-60 cells".ACTA PHARMACOLOGICA SINICA 21.8(2000):759-764.