Potentiation of T cell function by a marine algae-derived sulfated polymannuroguluronate: In vitro analysis of novel mechanisms

doi:10.1254/jphs.FPJ04026X

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Potentiation of T cell function by a marine algae-derived sulfated polymannuroguluronate: In vitro analysis of novel mechanisms
	Xia, W ; Li, J ; Geng, MY; Xin, XL ; Ding, J
刊名	JOURNAL OF PHARMACOLOGICAL SCIENCES
	2005-01
卷号	97 期号:1 页码:107-115
关键词	sulfated polymannuroguluronate immunomodulation T lymphocytes cytokine
ISSN号	1347-8613
DOI	10.1254/jphs.FPJ04026X
文献子类	Article
英文摘要	Marine algae-derived sulfated polymannuroguluronate (SPMG), a candidate drug for AIDS treatment, was intraperitoneally injected into normal mice for 6 weeks, and the in vivo and in vitro mechanisms of SPMG for immunomodulation were investigated in isolated lymphocytes by MTT assay, flow cytometry, and surface plasmon resonance assay. SPMG treatment at 5 and 10 mg/kg enhanced concanavalin A (ConA)-induced T cell proliferation, cellular levels of CD69, interleukin-2 (IL-2), and interferon-gamma(IFN-gamma), as well as CD4/CD8 ratio, while decreasing tumor necrosis factor-alpha (TNF-alpha) level in T cells of peripheral blood mononuclear cells. In addition, 1 molecule of SPMG bound to 2/3 molecules of IL-2 with a K-D of 9.53 x 10(-7) M. Heparin prevented SPMG binding to IL-2 by 72.2%; thus, to a large extent, SPMG and heparin share common binding sites on IL-2. In contrast, other glycosaminoglycans (e.g., chondroitin sulfate and dermatan sulfate) had little effect on SPMG and IL-2 interaction, suggesting the requirement of a defined sequence within the sugar chain for specific recognition of IL-2. Concomitant treatment of IL-2 and SPMG augmented lymphocyte proliferation, compared with IL-2 alone; in contrast, SPMG alone had no proliferative effect. Taken together, our findings demonstrated for the first time that SPMG exerted its immunomodulation by direct activation of T cell function, accompanied by simultaneous modulation of cytokine function, which suggests that SPMG would show great promise for use in anti-AIDS therapy.
WOS关键词	ACANTHOPANAX-OBOVATUS ROOTS ; ANTI-HIV ACTIVITY ; IFN-GAMMA ; LYMPHOCYTE SUBSETS ; CD69 EXPRESSION ; FLOW-CYTOMETRY ; TNF-ALPHA ; CYTOKINE ; POLYSACCHARIDE ; RESPONSES
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	JAPANESE PHARMACOLOGICAL SOC
WOS记录号	WOS:000226635000017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273966]
专题	药理学第一研究室
通讯作者	Geng, MY
作者单位	1.Ocean Univ China, Dept Pharmacol, Marine Drug & Food Inst, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Xia, W,Li, J,Geng, MY,et al. Potentiation of T cell function by a marine algae-derived sulfated polymannuroguluronate: In vitro analysis of novel mechanisms[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2005,97(1):107-115.
APA	Xia, W,Li, J,Geng, MY,Xin, XL,&Ding, J.(2005).Potentiation of T cell function by a marine algae-derived sulfated polymannuroguluronate: In vitro analysis of novel mechanisms.JOURNAL OF PHARMACOLOGICAL SCIENCES,97(1),107-115.
MLA	Xia, W,et al."Potentiation of T cell function by a marine algae-derived sulfated polymannuroguluronate: In vitro analysis of novel mechanisms".JOURNAL OF PHARMACOLOGICAL SCIENCES 97.1(2005):107-115.